Evaluation of allelopathic potential and quantification of momilactone A,B from rice hull extracts and assessment of inhibitory bioactivity on paddy field weeds
Evaluation of allelopathic potential and quantification of momilactone A,B from rice hull extracts and assessment of inhibitory bioactivity on paddy field weeds
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DOI：
10.1021/jf052796x
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发表时间：
2006-04-05
影响因子：
6.1
通讯作者：
Kim, SH
中科院分区：
文献类型：
--
作者：
Chung, IM;Kim, JT;Kim, SH
水稻（Oryza sativa L.）利用水稻船体浸提液对稗草（Echinochloa crus-galli P. Beauv.）变种测定船体种质中豆蔻内酯A和B的含量，并评价豆蔻内酯A和B作为水稻田除草剂的潜在天然来源的抑制活性。稗草等四种杂草。变种Oryzicola Ohwi、鸭舌草Monochoria vagulovar.在稻田中试验了车前草、灯芯草和黑口马蹄。在99个水稻品种中，包括诺因达里在内的前5个品种的平均干重抑制率（AIDW）均大于50%。其中Noindari的作用最强（55.6%）。排名中的下五个显示出大于40%的抑制效果。抑制率在20 ~ 40%之间的品种有46个，抑制率大于10%的品种有29个。有14个品种的抑菌效果很低（低于10%），其中最低的是Heunbe（4.7%）。结果表明，这些品种对种子发芽率、发芽率、叶干重、秸秆干重和根干重的平均抑制率分别为19.8%、9.9%、16.6%、38.9%和26.8%。根据水稻品种的全乳内酯含量（乳内酯A +乳内酯B），将水稻品种分为6类。在Baekna水稻品种中发现了最高水平的豆蔻内酯A（34.7 μ g g（-1）），并且Baekgwangok含有最高水平的豆蔻内酯B（37.8 μ g g（-1））。在化感作用潜力与遗传特性和形态特征方面，总抑制率（TIR）朝鲜稻品种为18.3%，中熟品种为19.0%，无色船体品种为17.8%，芒品种为18.3%，无色芒品种为19.0%。此外，韩国品种表现出更高的TM（4.5 μ g g（-1）），与晚熟品种（4.4 μ g g（-1））相比，有无色壳（4.1 μ g（-1）），芒（4.7 μ g（-1）），和无色芒（4.8 μ g（-1））。豆蔻内酯A水平通常高于豆蔻内酯B水平。对稗草的总抑制率与平均发芽抑制率（AIG）（r（2）= 0.62***）、平均发芽抑制率（AIDW）（r（2）= 0.92***）相关，并受GP抑制率的影响（r（2）= 0.57***）。水稻品种的产地与平均生长势呈显著正相关（r（2）= -0.23*），成熟期与干重和干重呈正相关（r（2）= 0.15** 和0.19**）。此外，罗汉果内酯含量还与产地（r（2）= -0.32*）、成熟时间（r（2）= 0.13*）和芒数有关（毛内酯A，r（2）= 0.23*; momilactone B，r（2）= 0.14**），表明有芒水稻品种，韩国品种，成熟时间较晚的品种含有较高水平的豆蔻内酯。此外，还研究了毛霉内酯A和B的生物活性（0、250、500、1000、2000和4000 g a.i./ ha）对水稻田杂草的防效试验结果表明，豆蔻内酯A和B对水稻无毒性，且豆蔻内酯A对水稻田杂草的抑制活性高于豆蔻内酯B。当与不含毛霉内酯的对照相比时，对E crus-galli P. Beauv.在施用豆蔻内酯A后第14天出现稻瘟病菌。抑制作用随着化合物浓度从250至4000 g a.i./ ha.此外，苦杏仁内酯A对E的抑制作用大于苦杏仁内酯B。粗皮鸡变种oryzicola Ohwi、稻蚜M.瓦格拉湾plantagelae、S. juncoides和E.黑口外在阔叶杂草中，豆蔻内酯A表现出最高的抑制效果（在4000 g a.i./ ha）在S.与无毛内酯对照相比，施用后14天，Juncoides。利用水稻品种的遗传特性和形态特征，进一步研究水稻品种的化感作用和苦皮内酯，将有助于培育具有化感潜力的水稻品种。稻壳中存在的Momilactones A和B可能作为一种潜在的天然除草剂源用于稻田杂草控制，减少对合成除草剂的依赖。
Rice (Oryza sativa L.) hull extracts were used in a bioassay to evaluate the allelopathic potential of rice on the germination and growth of barnyard grass (Echinochloa crus-galli P. Beauv. var. oryzicola Ohwi), to quantify momilactone A and B levels in rice hull germplasm and to assess the inhibitory bioactivity of momilactone A and B as a potential natural source of herbicide for weed control in paddy fields. Four varieties of weeds including E crus-galli P. Beauv. var. oryzicola Ohwi, Monochoria vaginalis var. plantaginea, Scirpus juncoides, and Eleocharis kuroguwai were tested in the paddy field. Of 99 rice varieties, the top five including Noindari exhibited inhibition effects greater than 50% in average inhibition of dry weight (AIDW). Noindari among them exerted the strongest effect (55.6%). The next five in the ranking exhibited inhibition effects of greater than 40%. Also, 46 varieties had inhibition effects between 20 and 40%, and 29 varieties had inhibition effects greater than 10%. Fourteen varieties had very low inhibitory effects (less than 10%), the lowest of which was Heunbe (4.7%). These varieties showed a mean inhibition of 19.8% for germination rate (GR), 9.9% for germination percentage (GP), 16.6% for leaf dry weight (LDW), 38.9% for straw dry weight (SDW), and 26.8% for root dry weight (RDW). Rice varieties were classified into six categories based on their total momilactones (TMs) (momilactone A + momilactone B). The highest level of momilactone A was found in the Baekna rice variety (34.7 mu g g(-1)), and Baekgwangok contained the highest level of momilactone B (37.8 mu g g(-1)). In allelopathic potential with genetic properties and morphological characteristics, the total inhibition rate (TIR) was 18.3% for Korean rice varieties, 19.0% for middle maturing varieties, 17.8% for colorless hull varieties, 18.3% for awn varieties, and 19.0% for colorless awn varieties. In addition, Korean varieties showed higher TMs (4.5 mu g g(-1)) as compared with varieties that were late maturing (4.4 mu g g(-1)), had colorless hulls (4.1 mu g g(-1)), awns (4.7 mu g g(-1)), and colorless awns (4.8 mu g g(-1)). Momilactone A levels were generally higher than momilactone B levels. Total inhibition rates on barnyard grass correlated with average inhibitions of germination (AIG) (r(2) = 0.62***), AIDW (r(2) = 0.92***), and were affected by the inhibition rate of GP (r(2) = 0.57***). Regions of origin for rice varieties correlated with the AIG (r(2) = -0.23***), and maturing time showed a positive correlation with SDW (r(2) = 0.15**) and RDW (r(2) = 0.19**). Levels of momilactones were also correlated with the region of origin (r(2) = -0.32***), maturing time (r(2) = 0.13***), and awns (momilactone A, r(2) = 0.23***; momilactone B, r(2) = 0.14**), suggesting that rice varieties with awns, Korean varieties, and varieties with later maturing times contain higher levels of momilactone. Also, the investigation of the momilactone A and B bioactivity (0, 250, 500, 1000, 2000, and 4000 g a.i./ha) for weed control in paddy fields indicated that momilactones A and B exhibited no toxicity (0 in all concentrations) against rice plants, and the inhibitory bioactivity on weeds with momilactone A was higher than that of momilactone B. When compared with no momilactone control, the highest inhibitory effect (50%) on E crus-galli P. Beauv. var.oryzicola Ohwi was shown on the 14th day after th application of momiactone A. The inhibitory effect increased with the concentration of the compound from 250 to 4000 g a.i./ha. Furthermore, momilactone A showed greater suppression than momliactone B toward toward E. crus-galli P. Beauv. var. oryzicola Ohwi, M. vaginalis var. plantaginea, S. juncoides, and E. kuroguwai. In broad weed species, momilactone A showed the highest inhibitory effect (90% in4000 g a.i./ha) on S. juncoides, 14 days after the application as compared with no momilactone control. Further studies on allelopathic effects and momilactones from the germplasm of rice varieties using genetic properties and morphological characteristics may facilitate the development of rice varieties with higher allelopathic potential. Momilactones A and B present in rice hulls may serve as a potential natural herbicide source for weed control in paddy fields reducing the dependence on synthetic herbicides.