Evolution of prothrombin: isolation and characterization of the cDNAs encoding chicken and hagfish prothrombin.
Evolution of prothrombin: isolation and characterization of the cDNAs encoding chicken and hagfish prothrombin.
复制标题
凝血酶原的进化：编码鸡和盲鳗凝血酶原的 cDNA 的分离和表征。
DOI：
10.1007/bf00166164
复制
发表时间：
1994
影响因子：
3.9
通讯作者：
MacGillivray,RT
中科院分区：
文献类型：
--
作者：
Banfield,DK;Irwin,DM;Walz,DA;MacGillivray,RT
测定了鸡和盲鳗凝血酶原的cDNA序列。序列预测，从这两个物种的凝血酶原合成为一个prepro-protein组成的推定Gla域，两个kringle域，和一个双链蛋白酶域。鸡和盲鳗凝血酶原共享51.6%的氨基酸序列同一性（313/627残基）。鸡和盲鳗的凝血酶原在结构上与人、牛、大鼠和小鼠的凝血酶原非常相似，并且所有六个物种共享41%的氨基酸序列同一性。人、牛、大鼠、小鼠、鸡和盲鳗凝血酶原的氨基酸序列比对表明，凝血酶B链和前肽-Gla结构域是脊椎动物凝血酶原的共同功能最受限制的区域。
The cDNA sequences of chicken and hagfish prothrombin have been determined. The sequences predict that prothrombin from both species is synthesized as a prepro-protein consisting of a putative Gla domain, two kringle domains, and a two-chain protease domain. Chicken and hagfish prothrombin share 51.6% amino acid sequence identity (313/627 residues). Both chicken and hagfish prothrombin are structurally very similar to human, bovine, rat, and mouse prothrombin and all six species share 41% amino acid sequence identity. Amino acid sequence alignments of human, bovine, rat, mouse, chicken, and hagfish prothrombin suggest that the thrombin B-chain and the propeptide-Gla domain are the regions most constrained for the common function(s) of vertebrate prothrombins.