Design, fabrication and in vitro evaluation of a novel polymer-hydrogel hybrid scaffold for bone tissue engineering
Design, fabrication and in vitro evaluation of a novel polymer-hydrogel hybrid scaffold for bone tissue engineering
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DOI：
10.1002/term.1506
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发表时间：
2014-02-01
影响因子：
3.3
通讯作者：
Nukavarapu, Syam P.
中科院分区：
文献类型：
--
作者：
Igwe, John C.;Mikael, Paiyz E.;Nukavarapu, Syam P.
骨机械相容性和骨诱导性支架的开发对于骨组织工程应用，特别是对于大面积临界尺寸骨缺损的修复和再生是重要的。虽然以前的研究与承重支架已经显示出有希望的结果，有一个明确的需要开发更好的骨诱导策略，有效的支架为基础的骨再生。在这项研究中，我们设计并制造了一种新型的聚合物-水凝胶混合支架系统，其中承重聚合物基质和肽水凝胶允许在单个支架中协同结合机械强度和骨诱导性的巨大潜力。混合支架系统促进前成骨细胞增殖增加。此外，我们生物素化人重组骨形态发生蛋白2（rhBMP 2），并表征了生物素的添加及其对rhBMP 2生物活性的影响。使用生物素-链霉亲和素复合物将生物素化的rhBMP 2拴系到杂交支架上。控释研究表明，用栓系的rhBMP 2杂合支架组增加了rhBMP 2保留。用大鼠骨髓基质细胞和小鼠前成骨细胞系MC 3 T3-E1细胞进行混合支架的体外评价。碱性磷酸酶（ALP）、胶原I（Col I）、骨桥蛋白（OPN）、骨唾液蛋白（BSP）、Runx-2和骨钙素（OC）的基因表达在接种在rhBMP 2栓系的杂交支架上的MC 3 T3-E1细胞中比未栓系的对应物增加，证明了杂交移植物的骨诱导潜力。这些研究结果表明，开发一种新的聚合物-水凝胶混合系统，是承重和骨诱导有效的骨组织工程的可能性。版权所有（c）2012约翰威利父子有限公司
The development of a bone mechanically-compatible and osteoinductive scaffold is important for bone tissue engineering applications, particularly for the repair and regeneration of large area critically-sized bone defects. Although previous studies with weight-bearing scaffolds have shown promising results, there is a clear need to develop better osteoinductive strategies for effective scaffold-based bone regeneration. In this study, we designed and fabricated a novel polymer-hydrogel hybrid scaffold system in which a load-bearing polymer matrix and a peptide hydrogel allowed for the synergistic combination of mechanical strength and great potential for osteoinductivity in a single scaffold. The hybrid scaffold system promoted increased pre-osteoblastic cell proliferation. Further, we biotinylated human recombinant bone morphogenetic protein 2 (rhBMP2), and characterized the biotin addition and its effect on rhBMP2 biological activity. The biotinylated rhBMP2 was tethered to the hybrid scaffold using biotin-streptavidin complexation. Controlled release studies demonstrated increased rhBMP2 retention with the tethered rhBMP2 hybrid scaffold group. In vitro evaluation of the hybrid scaffold was performed with rat bone marrow stromal cells and mouse pre-osteoblast cell line MC3T3-E1 cells. Gene expression of alkaline phosphatase (ALP), collagen I (Col I), osteopontin (OPN), bone sialoprotein (BSP), Runx-2 and osteocalcin (OC) increased in MC3T3-E1 cells seeded on the rhBMP2 tethered hybrid scaffolds over the untethered counterparts, demonstrating osteoinductive potential of the hybrid graft. These findings suggest the possibility of developing a novel polymer-hydrogel hybrid system that is weight bearing and osteoinductive for effective bone tissue engineering. Copyright (c) 2012 John Wiley & Sons, Ltd.