TIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity
TIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity
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DOI：
10.1126/science.abq8180
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发表时间：
2022-07-29
期刊：
影响因子：
56.9
通讯作者：
Chai, Jijie
中科院分区：
文献类型：
--
作者：
Jia, Aolin;Huang, Shijia;Chai, Jijie
通过具有N-末端Toll/白细胞介素-1受体（TIR）结构域的核苷酸结合富含亮氨酸重复序列（NLR）受体的植物病原体激活的免疫信号传导会聚于增强的疾病易感性1（EDS 1）及其直接配偶体，植物抗毒素缺陷4（PAD 4）或衰老相关基因101（SAG 101）。TIR编码的烟酰胺腺嘌呤二核苷酸水解酶（NADase）产生信号分子以促进EDS 1-PAD 4和EDS 1-SAG 101与辅助NLR亚类的排他性相互作用。在这项工作中，我们表明，TIR蛋白催化腺苷二磷酸（ADP）核糖基化的三磷酸腺苷（ATP）和ADP核糖（ADPR）通过ADPR聚合酶和NADase活性，形成ADP核糖基化的ATP（ADPr-ATP）和ADPr-ADPR（di-ADPR），分别。ADPr-ATP或二-ADPR的特异性结合在体外和植物中变构促进EDS 1-SAG 101与辅助NLR N需求基因1A（NRG 1A）的相互作用。我们的数据揭示了TIR的酶活性，其能够特异性激活EDS 1-SAG 101-NRG 1免疫分支。
Plant pathogen-activated immune signaling by nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain converges on Enhanced Disease Susceptibility 1 (EDS1) and its direct partners, Phytoalexin Deficient 4 (PAD4) or Senescence-Associated Gene 101 (SAG101). TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) produces signaling molecules to promote exclusive EDS1-PAD4 and EDS1-SAG101 interactions with helper NLR subclasses. In this work, we show that TIR-containing proteins catalyze adenosine diphosphate (ADP)-ribosylation of adenosine triphosphate (ATP) and ADP ribose (ADPR) through ADPR polymeraselike and NADase activity, forming ADP-ribosylated ATP (ADPr-ATP) and ADPr-ADPR (di-ADPR), respectively. Specific binding of ADPr-ATP or di-ADPR allosterically promotes EDS1-SAG101 interaction with helper NLR N requirement gene 1A (NRG1A) in vitro and in planta. Our data reveal an enzymatic activity of TIRs that enables specific activation of the EDS1-SAG101-NRG1 immunity branch.