ANIMAL-CELLS DEPENDENT ON EXOGENOUS PHOSPHATIDYLCHOLINE FOR MEMBRANE BIOGENESIS
ANIMAL-CELLS DEPENDENT ON EXOGENOUS PHOSPHATIDYLCHOLINE FOR MEMBRANE BIOGENESIS
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DOI：
10.1073/pnas.79.6.1698
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
RAETZ, CRH
中科院分区：
文献类型：
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作者：
ESKO, JD;NISHIJIMA, M;RAETZ, CRH
中国仓鼠卵巢细胞(CHO)突变体(品系58)，CDP-胆碱合成酶(磷酸胆碱胞苷酰转移酶；CTP：在非许可条件下的磷酸胆碱胞苷酰胆碱[Esko of Raetz 1980]有缺陷。向培养基中添加40μM卵磷脂酰胆碱或溶血磷脂酰胆碱抑制了细胞的温度敏感性。突变体 58 并允许具有不同极性头基的磷脂、脂蛋白结合磷脂、鞘磷脂和甘油磷酸胆碱不支持在 40°C 下长时间生长；磷脂酰二甲基乙醇胺、D-磷脂酰胆碱和 β-磷脂酰胆碱是相当有效的。饱和磷脂酰胆碱，尤其是脂肪酸长度为 12-18 Ca 的磷脂酰胆碱，抑制表型。含有醚连接烃的磷脂是无效的；在表型旁路条件下，突变体剩余的内源性磷脂酰胆碱合成不受刺激，但与野生型相比，外源性脂质的摄取明显增强。磷脂可以提供动物细胞膜生物发生所需的至少50%的磷脂酰胆碱，并且可以调节外源磷脂的摄取。
A Chinese hamster ovary cell (CHO) mutant (strain 58), defective in CDP-choline synthetase (cholinephosphate cytidylyltransferase; CTP:cholinephosphate cytidylylcholine under nonpermissive conditions [Esko of Raetz 1980]. The addition of 40 .mu.M egg phosphatidylcholine or lysophosphatidylcholine to the medium suppresses the temperature sensitivity of mutant 58 and permits the growth of colonies at the restrictive temperature. Phospholipids with different polar headgroups, lipoprotein-bound phospholipids, sphingomyelin, and glycerophosphocholine do not support prolonged growth at 40.degree. C; phosphatidylcholine analogs such as phosphatidyldimethylethanolamine, D-phosphatidylcholine, and .beta.-phosphatidylcholine are quite effective. A broad range of saturated phosphatidylcholines, especially those with fatty acids 12-18 Ca in length, suppresses the phenotype. Phospholipids containing ether-linked hydrocarbons are ineffective; polyunsaturated phosphatidylcholines are toxic. Residual endogenous synthesis of phosphatidylcholine by the mutant is not stimulated under conditions of phenotypic bypass, but the uptake of exogenous lipid is enhanced considerably compared to the wild type. Apparently, exogenous phospholipid can provide at least 50% of the phosphatidylcholine required for membrane biogenesis in animal cells and that uptake of exogenous phospholipids may be regulated.