Distribution of the high-affinity choline transporter in the central nervous system of the rat
Distribution of the high-affinity choline transporter in the central nervous system of the rat
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DOI：
10.1016/s0306-4522(01)00147-6
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Haga, T
中科院分区：
文献类型：
--
作者：
Misawa, H;Nakata, K;Haga, T
在胆碱能神经末梢，依赖于Na+和Cl-，对半羟基-3敏感，高亲和力的胆碱摄取被认为是乙酰胆碱合成的限速步骤。负责该活性的高亲和力胆碱转运蛋白基因最近被克隆。在这里，我们报告了一种针对高亲和力胆碱转运蛋白的高度特异性抗体的产生和该蛋白在大鼠中枢神经系统中的分布。该抗体对几乎所有已知的胆碱能神经元及其终末区域进行染色。在嗅结节、基底前脑复合体、纹状体、桥中核、复合体、内侧缰核、颅神经运动核、脊髓腹角和中间带均可见高亲和力胆碱转运蛋白免疫反应阳性胞体。值得注意的是，高亲和力胆碱转运蛋白阳性轴突纤维和点状突起在大脑皮层、海马体、杏仁核、纹状体、几个丘脑核团和脑干等许多脑区均可见到。切断舌下神经导致同侧舌下运动核内神经元高亲和力胆碱转运体免疫反应性的丧失，这与胆碱乙酰转移酶的免疫反应性丧失是平行的。这些结果证实了高亲和力胆碱转运蛋白在胆碱能神经元中唯一表达，并有效地转运到轴突终末。该抗体可用于研究人和啮齿动物在不同病理条件下胆碱能细胞体和轴突终末的可能变化。(C)2001年IBRO。爱思唯尔科学有限公司出版。版权所有。
In cholinergic nerve terminals, Na+- and Cl--dependent, hemicholinium-3-sensitive, high-affinity choline uptake is thought to be the rate-limiting step in acetylcholine synthesis. The hi.-h-affinity choline transporter cDNA responsible for the activity was recently cloned. Here we report production of a highly specific, antibody to the high-affinity choline transporter and distribution of the protein in the CNS of the rat. The antibody stained almost all known cholinergic neurons and their terminal fields. High-affinity choline transporter-immunoreactive cell bodies were demonstrated in the olfactory tubercle, basal forebrain complex, striatum, mesopontine, complex, medial habenula, cranial nerve motor nuclei, and ventral horn and intermediate zone of the spinal cord. Noticeably, high densities of high-affinity choline transporter-positive axonal fibers and puncta were encountered in many brain regions such as cerebral cortex, hippocampus, amygdala, striatum, several thalamic nuclei, and brainstem. Transection of the hypoglossal nerve resulted in a loss of high-affinity choline transporter immunoreactivity in neurons within the ipsilateral hypoglossal motor nucleus, which paralleled a loss of immunoreactivity to choline acetyltransferase. The antibody also stained brain sections from human and mouse, suggesting cross-reactivity.These results confirm that the high-affinity choline transporter is uniquely expressed in cholinergic neurons and is efficiently transported to axon terminals. The antibody will be useful to investigate possible changes in cholinergic cell bodies and axon terminals in human and rodents under various pathological conditions. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.