Evaluation of a cefoxitin 30 microg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus.
Evaluation of a cefoxitin 30 microg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus.
复制标题
对 Iso-Sensitest 琼脂上的头孢西丁 30 微克圆片用于检测耐甲氧西林金黄色葡萄球菌的评估。
DOI：
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发表时间：
2003
影响因子：
5.2
通讯作者：
G. Kahlmeter
中科院分区：
文献类型：
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作者：
R. Skov;R. Smyth;M. Clausen;A. Larsen;N. Frimodt;B. Olsson;G. Kahlmeter
目标
为了评估头孢西丁 30 微克圆片在 Iso-Sensitest 琼脂上的性能，使用半汇合接种物并在 35-36°C 下孵育过夜，用于检测耐甲氧西林金黄色葡萄球菌 (MRSA)。
方法
使用半汇合接种物并在 35-36 摄氏度下过夜孵育，用头孢西丁 30 微克圆盘在 Iso-Sensitest 琼脂上测试了总共 457 株金黄色葡萄球菌，包括几种定义的 PFGE 类型的 190 株 MRSA 和一些低水平耐药菌株。该方法与标准 SRGA（瑞典抗生素参考组）方法进行了比较 （苯唑西林 1 微克圆片置于补充有 5% 去纤维马血的 Iso-Sensitest 琼脂上，汇合生长并在 30 摄氏度环境空气中孵育 24 小时）。
结果
头孢西丁方法非常出色，使用 S > 或 = 29 mm 且 R < 29 mm 的解释区直径，灵敏度为 100%，特异性为 99%。它的性能比 SRGA 方法好得多，SRGA 方法在使用当前 S > 或 = 12 mm 的断点时，对于困难菌株的集合，其灵敏度仅为 78%。
结论
我们建议头孢西丁方法应取代 SRGA 目前推荐的 MRSA 检测方法，并且该方法非常适合 BSAC 方法。
OBJECTIVES
To evaluate the performance of a cefoxitin 30 microg disc on Iso-Sensitest agar, using a semi-confluent inoculum and overnight incubation at 35-36 degrees C, for detection of methicillin-resistant Staphylococcus aureus (MRSA).
METHODS
A total of 457 S. aureus, including 190 MRSA of several defined PFGE types and a number of low-level resistant isolates, were tested with a cefoxitin 30 microg disc on Iso-Sensitest agar, using a semi-confluent inoculum and overnight incubation at 35-36 degrees C. This method was compared with the standard SRGA (Swedish Reference Group for Antibiotics) method (oxacillin 1 microg disc on Iso-Sensitest agar supplemented with 5% defibrinated horse blood, confluent growth and 24 h incubation in ambient air at 30 degrees C).
RESULTS
The cefoxitin method was excellent, with a sensitivity of 100% and a specificity of 99% using an interpretative zone diameter of S > or = 29 mm and R < 29 mm. Its performance was much better than the SRGA method, which with this collection of difficult strains had a sensitivity of only 78% using the current breakpoint of S > or = 12 mm.
CONCLUSION
We suggest that the cefoxitin method should replace that currently recommended by the SRGA for the detection of MRSA, and that it would fit well into BSAC methodology.