EPA and DHA requirements in early juvenile Red Sea bream using HUFA enriched Artemia Nauplii

EPA and DHA requirements in early juvenile Red Sea bream using HUFA enriched Artemia Nauplii
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DOI:
10.2331/fishsci.62.246
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发表时间:
1996-04-01
期刊:
影响因子:
1.9
通讯作者:
Watanabe, T
Watanabe, T
中科院分区:
农林科学4区
文献类型:
--
作者:
Furuita, H;Takeuchi, T;Watanabe, T

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采用不同浓度的二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对真鲷(Pagrus major)幼鱼进行为期12 d的饲养试验,研究真鲷幼鱼对EPA和DHA的需要量。摄食富含BPA的卤虫的幼体存活率高,但活力很差,而摄食富含DHA的卤虫的幼体存活率和活力都很高。提高卤虫体内DHA水平能有效提高鱼类的活力。在DHA富集的卤虫中,EPA含量从初始值增加,同时出现二十二碳五烯酸(DPA),表明DHA通过DPA在无节幼体中逆转化为EPA。以活力为指标,估算出红鲷幼鱼对DHA的需要量为卤虫干物质的0.95-1.62%,以成活率为指标,红鲷幼鱼对EPA和DHA的需要量分别为2.25%和0.95%(n-3 HUFA为2.05%)。
Feeding experiments were conducted to determine the requirements of early juvenile red sea bream (Pagrus major) for eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) by feeding Artemia nauplii containing different levels of EPA or DKA for 12 days. Juveniles fed BPA-enriched Artemia had a high survival rate but very poor vitality, while those fed on Artemia enriched with DHA had both a high survival rate and vitality. The vitality of fish was effectively improved by elevation of DHA levels in Artemia. In the DHA-enriched Artemia, the EPA content increased from the initial value together with the appearance of docosapentaenoic acid (DPA), indicating retroconversion from DHA to EPA through DPA in the nauplii. The requirement of early juvenile red sea bream for DHA was estimated to be 0.95-1.62% in Artemia on a dry matter basis when vitality was used as an index, and was satisfied with 2.25% EPA or 0.95% DHA (2.05% n-3 HUFA) on a dry basis when based on the survival rate.