Expression of the Otx2 homeobox gene in the developing mammalian brain: embryonic and adult expression in the pineal gland.
Expression of the Otx2 homeobox gene in the developing mammalian brain: embryonic and adult expression in the pineal gland.
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Otx2 同源盒基因在发育中的哺乳动物大脑中的表达：胚胎和成人在松果体中的表达。
DOI：
10.1111/j.1471-4159.2006.03773.x
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发表时间：
2006
影响因子：
4.7
通讯作者：
Moller,Morten
中科院分区：
文献类型：
--
作者：
Rath,MartinF;Munoz,Estela;Ganguly,Surajit;Morin,Fabrice;Shi,Qiong;Klein,DavidC;Moller,Morten
Otx2 是一种脊椎动物同源盒基因，已被发现对于大脑头端区域的发育至关重要，并且似乎在视网膜感光细胞和松果体细胞的发育中发挥作用。在这项研究中，揭示了 Otx2 在大鼠大脑中的时间表达模式，特别强调整个胚胎晚期和出生后阶段的松果体。 Otx2在胚胎脑中广泛的高表达在成人中逐渐局限于松果体。Crx（锥杆同源盒）是Otx2的下游靶基因，其松果体表达模式与Otx2相似，尽管在发病时间上有明显的滞后。在松果体提取物中鉴定出 Otx2 蛋白，并发现其位于松果体细胞中。松果体总 Otx2 mRNA 没有表现出昼夜变化，也不受交感神经输入去除的影响，表明 Otx2 mRNA 水平似乎与腺体的光神经输入无关。我们的结果与Otx2的松果体表达是发育所必需的观点一致，并且我们假设它在成人中在控制与光转导和褪黑激素合成相关的基因簇的表达中发挥作用。
Otx2is a vertebrate homeobox gene, which has been found to be essential for the development of rostral brain regions and appears to play a role in the development of retinal photoreceptor cells and pinealocytes. In this study, the temporal expression pattern ofOtx2was revealed in the rat brain, with special emphasis on the pineal gland throughout late embryonic and postnatal stages. Widespread high expression ofOtx2in the embryonic brain becomes progressively restricted in the adult to the pineal gland.Crx(cone–rod homeobox), a downstream target gene of Otx2, showed a pineal expression pattern similar to that ofOtx2, although there was a distinct lag in time of onset. Otx2 protein was identified in pineal extracts and found to be localized in pinealocytes. Total pineal Otx2 mRNA did not show day–night variation, nor was it influenced by removal of the sympathetic input, indicating that the level of Otx2 mRNA appears to be independent of the photoneural input to the gland. Our results are consistent with the view that pineal expression ofOtx2is required for development and we hypothesize that it plays a role in the adult in controlling the expression of the cluster of genes associated with phototransduction and melatonin synthesis.