mRNA sequence features that contribute to translational regulation in Arabidopsis.
mRNA sequence features that contribute to translational regulation in Arabidopsis.
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DOI：
10.1093/nar/gki240
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发表时间：
2005
影响因子：
14.9
通讯作者：
Bailey-Serres J
中科院分区：
文献类型：
--
作者：
Kawaguchi R;Bailey-Serres J
DNA 微阵列用于评估在控制生长条件下和轻度脱水胁迫 (DS) 后拟南芥叶片中多核糖体复合物中单个 mRNA 种类的比例的调节。分析确定多核糖体中单个基因转录物的百分比（核糖体负载）范围从超过 95% 到 <5%。 DS 导致核糖体负载量从 82% 减少至 72%，同时超过 60% 的 mRNA 保持多核糖体关联，且丰度增加。为了确定负责翻译调节的序列特征，比较了核糖体装载值和全长 mRNA 序列的特征。 5'-非翻译区 (5'-UTR) 中具有极端长度或高 GU 含量的 mRNA 通常翻译效果较差。 DS 下，5'-UTR 中 GC 含量高且开放阅读框长的 mRNA 的核糖体负载量显着受损。对起始 A+1UG 密码子背景的评估揭示了具有不同核糖体加载值的 mRNA 中核苷酸 -10 至 -1（尤其是 -4 和 -3）中腺嘌呤频率的差异。值得注意的是，有助于翻译调节的 mRNA 特征无法完全解释核糖体负载的变化，表明有其他因素有助于拟南芥中的翻译调节。
DNA microarrays were used to evaluate the regulation of the proportion of individual mRNA species in polysomal complexes in leaves of Arabidopsis thaliana under control growth conditions and following a mild dehydration stress (DS). The analysis determined that the percentage of an individual gene transcript in polysomes (ribosome loading) ranged from over 95 to <5%. DS caused a decrease in ribosome loading from 82 to 72%, with maintained polysome association for over 60% of the mRNAs with an increased abundance. To identify sequence features responsible for translational regulation, ribosome loading values and features of full-length mRNA sequences were compared. mRNAs with extreme length or high GU content in the 5′-untranslated regions (5′-UTRs) were generally poorly translated. Under DS, mRNAs with both a high GC content in the 5′-UTR and long open reading frame showed a significant impairment in ribosome loading. Evaluation of initiation A+1UG codon context revealed distinctions in the frequency of adenine in nucleotides −10 to −1 (especially at −4 and −3) in mRNAs with different ribosome loading values. Notably, the mRNA features that contribute to translational regulation could not fully explain the variation in ribosome loading, indicating that additional factors contribute to translational regulation in Arabidopsis.
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