Polysaccharide Peptide-Induced Virus Resistance Depends on Ca2+ Influx by Increasing the Salicylic Acid Content and Upregulating the Leucine-Rich Repeat Gene in Arabidopsis thaliana
Polysaccharide Peptide-Induced Virus Resistance Depends on Ca2+ Influx by Increasing the Salicylic Acid Content and Upregulating the Leucine-Rich Repeat Gene in Arabidopsis thaliana
复制标题
拟南芥中多糖肽诱导的病毒抗性依赖于 Ca2+ 内流，通过增加水杨酸含量和上调富含亮氨酸的重复基因来实现
DOI：
10.1094/mpmi-10-17-0242-r
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发表时间：
2018
影响因子：
3.5
通讯作者：
Wu Yunfeng
中科院分区：
文献类型：
--
作者：
Zhao Lei;Chen Yujia;Yang Wen;Zhang Yuanle;Chen Wenbao;Feng Chaohong;Wang Qaochun;Wu Yunfeng
植物病毒病给农业生产造成严重的经济损失。生物源抗病毒剂的开发为有效防治植物病毒病提供了一种新的策略。我们曾报道外源施用多糖肽（PSP）对烟草花叶病毒（Tobacco mosaic virus）的侵染有明显的抑制作用。在这项研究中，我们进一步详细研究了PSP诱导拟南芥抗病毒的机制。我们发现，PSP显着诱导Ca 2+内流，增加过氧化氢和水杨酸（SA）的积累在拟南芥细胞。通过RNA测序和基因缺失突变体的筛选，获得了一个含有白细胞介素1受体-核苷酸结合位点-富含亮氨酸重复结构域（LRR）的基因。thaliana. LRR基因缺失后，A.突变后，thalianato PSP显著减弱。LRR基因在N.本氏烟的SA含量和PR 1基因表达量。苯达米亚那显著增加。通过对LRR基因表达和A.用PSP和PSP+乙二醇-双（β-氨基乙基醚）-N，N'-四乙酸处理黄瓜花叶病毒，结果表明，PSP提高了黄瓜花叶病毒的抗性。thalianaby诱导Ca2+内流，随后提高LRR基因的表达，从而进一步增加SA含量。
Plant viral diseases cause severe economic losses in agricultural production. The development of biosource-derived antiviral agents provides an alternative strategy to efficiently control plant viral diseases. We previously reported that the exogenous application of polysaccharide peptide (PSP) exerts significant inhibitive effects onTobacco mosaic virusinfection inNicotiana tabacum. In this study, we studied in additional detail the mechanism by which PSP can induce virus resistance inArabidopsis thaliana. We found that PSP significantly induced Ca2+influx and increased the accumulation of hydrogen peroxide and salicylic acid (SA) in theA.thalianacells. A gene with a toll interleukin 1 receptor-nucleotide binding site-leucine-rich repeat domain (LRR) was obtained by RNA sequencing in combination with the screening of the gene-deletion mutants ofA. thaliana. TheLRRgene was deleted, and the inductive response ofA. thalianato PSP was significantly attenuated after mutation. After the heterologous overexpression of theLRRgene inN. benthamiana, the SA content andPR1gene expression inN. benthamianawere significantly increased. Through analyses of theLRRgene expression and the ability ofA. thalianato resistCucumber mosaic virusfollowing the treatments of PSP and PSP + ethyleneglycol-bis (beta-aminoethylether)-N,N’-tetraacetic acid, it was shown that PSP enhanced the virus resistance ofA. thalianaby inducing Ca2+influx and subsequently improving expression of theLRRgene, which further increased the SA content.