THE AMPLIFICATION OF THE COMPLETE MITOCHONDRIAL GENOME OF THE DAI-QU STOCK PSEUDOSCIAENA CROCEA AND THE DEVELOPMENT OF ITS DIAGNOSTIC PRIMERS
THE AMPLIFICATION OF THE COMPLETE MITOCHONDRIAL GENOME OF THE DAI-QU STOCK PSEUDOSCIAENA CROCEA AND THE DEVELOPMENT OF ITS DIAGNOSTIC PRIMERS
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发表时间：
2013
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通讯作者：
Shen Xi
中科院分区：
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作者：
Shen Xi
The complete mitochondrial DNA (mtDNA) genomes of Pseudosciaena crocea sampled from Min-yue and Dai-qu Stock were amplified by PCR and sequenced. The sequences of high variability regions from 25 samples respectively were obtained, which were divided into 3 Operational Taxonomic Units (OTUs): D1-N1, D1-N2 and D2. The results indicated that: all the Dai-qu Stock samples were clustered into D1-N1 type; Min-yue Stock samples were clustered into D1-N1, D1-N2 and D2 type. Primer pairs J1 and J2 were designed to significantly distinguish different OTUs, and PCR conditions were optimizated for identification. The results show that the accurate rate of identification for Min-yue Stock P. crocea is 100%, if the band pattern on photo belongs to D1-N2 or D2 type, and the accurate rate of identification for Dai-qu and Min-yue Stock P. crocea are respectively 86.2% and 13.8%, if it belongs to D1-N1 type, according to gel images of the samples. The research results provide technical support for varieties resources conservation, selective improved variety and market sales of the Dai-qu Stock P. crocea.
