Physiological and biochemical responses of brackish-water clam Corbicula japonica under global-warming conditions: Water temperature, salinity, and food availability
Physiological and biochemical responses of brackish-water clam Corbicula japonica under global-warming conditions: Water temperature, salinity, and food availability
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DOI：
10.1016/j.ecolind.2021.107866
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发表时间：
2021-10
影响因子：
6.9
通讯作者：
P. Pokhrel;Jumpei Suzuki;Shumona Akther;M. Fujita
中科院分区：
文献类型：
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作者：
P. Pokhrel;Jumpei Suzuki;Shumona Akther;M. Fujita
为了研究代表苦咸水小虾（clamCorbicula japonica）生理生化反应的生物标志物，我们进行了全因子设计实验，测试了不同的水温（20°C和25°C）、盐度（5和20 psu）和食物有效性（0.5和2.0 mg悬浮固体（SS）·ind - 1·d - 1）。水温升高显著降低了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性和氧自由基吸收能力（ORAC），导致脂质过氧化（即氧化损伤）。盐度激活或抑制这些生化标记。食物供应支持一种对抗氧化应激的解毒机制。主成分分析和聚类分析表明，与水温和/或盐度相关的8种实验条件共分为3类。从1组（水温20°C）到2组（水温25°C，盐度5 psu），状态指数、SOD、CAT和ORAC显著降低。随着进一步转向第三组（25°C水温和20 psu盐度），我们发现ORAC显著增加，导致氧化损伤，但没有死亡。我们的结论是，应该密切关注未来由全球变暖驱动的栖息地变化，特别是考虑到当地人为干扰会进一步加剧自然干扰。
To investigate biomarkers representing the physiological and biochemical responses of the brackish-water clamCorbicula japonica, we conducted a full factorial-design experiment to test different water-temperature levels (20 °C and 25 °C), salinity levels (5 and 20 psu), and food-availability levels (0.5 and 2.0 mg suspended solids (SS)·ind−1·d−1). Increase in water temperature significantly decreased superoxide dismutase (SOD) and catalase (CAT) activities and oxyradical-absorbance capacity (ORAC), leading to lipid peroxidation (i.e., oxidative damage). Salinity activated or inhibited these biochemical markers. Food availability supported a detoxification mechanism against oxidative stress. Principal-components and cluster analyses revealed that a total of eight experimental conditions fell into three groups related to water temperature and/or salinity. The shift from Group I (20 °C water temperature) to Group II (25 °C water temperature and 5-psu salinity) demonstrated that the condition index, SOD, CAT, and ORAC had significantly decreased. With the further shift to Group III (25 °C water temperature and 20-psu salinity), we found a prominent increase in ORAC, which led to oxidative damage but no mortality. We conclude that future habitat changes driven by global warming should be closely watched, particularly given that local anthropogenic disturbances further add to natural ones.