A Dof-CLE circuit controls phloem organization
A Dof-CLE circuit controls phloem organization
复制标题
DOI：
10.1038/s41477-022-01176-0
复制
发表时间：
2022-07
期刊：
影响因子：
18
通讯作者：
P. Qian;Wen Song;Miki Zaizen-Iida;Sawa Kume;Guodong Wang;Ye Zhang;Kaori Kinoshita-Tsujimura;J. Chai;T. Kakimoto
中科院分区：
文献类型：
--
作者：
P. Qian;Wen Song;Miki Zaizen-Iida;Sawa Kume;Guodong Wang;Ye Zhang;Kaori Kinoshita-Tsujimura;J. Chai;T. Kakimoto
韧皮部由筛分子（SE）和伴胞（CC）组成。在这里，我们表明，Dof类转录因子优先表达在韧皮部（韧皮部Dofs）不仅是必要的和足够的SE和CC分化，但也诱导韧皮部发育的负调节因子，CLAVATA 3/胚周围区域-EROUNDING REGION-RELATED 25（CLE 25），CLE 26和CLE 45分泌肽。克莱斯被几乎任何分生组织（BAM）类受体和CLAVATA 3不敏感受体激酶（CIK）共受体感知，转录后韧皮部Dof蛋白减少，SE和CC形成受到抑制。CLE-，BAM-或CIK类基因的多个突变引起SE和CC的异位形成，在每个韧皮部区域产生SE/CC簇。我们建议，虽然韧皮部Dofs诱导韧皮部细胞的形成，他们抑制过多的韧皮部细胞的形成诱导克莱斯。正常定位的SE和CC前体细胞似乎通过正反馈转录调节加强韧皮部Dofs的产生来克服克莱斯的作用。
The phloem consists of sieve elements (SEs) and companion cells (CCs). Here we show that Dof-class transcription factors preferentially expressed in the phloem (phloem-Dofs) are not only necessary and sufficient for SE and CC differentiation, but also induce negative regulators of phloem development, CLAVATA3/EMBRYO SURROUNDING REGION-RELATED25 (CLE25), CLE26 and CLE45 secretory peptides. CLEs were perceived by BARELY ANY MERISTEM (BAM)-class receptors and CLAVATA3 INSENSITIVE RECEPTOR KINASE (CIK) co-receptors, and post-transcriptionally decreased phloem-Dof proteins and repressed SE and CC formation. Multiple mutations in CLE-, BAM- or CIK-class genes caused ectopic formation of SEs and CCs, producing an SE/CC cluster at each phloem region. We propose that while phloem-Dofs induce phloem cell formation, they inhibit excess phloem cell formation by inducing CLEs. Normal-positioned SE and CC precursor cells appear to overcome the effect of CLEs by reinforcing the production of phloem-Dofs through a positive feedback transcriptional regulation.