High-level expression of the ribosomal protein L19 in human breast tumors that overexpress erbB-2.
High-level expression of the ribosomal protein L19 in human breast tumors that overexpress erbB-2.
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发表时间：
1993-03
期刊：
影响因子：
11.2
通讯作者：
Henry Jl;Coggin Dl;King Cr
中科院分区：
文献类型：
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作者：
Henry Jl;Coggin Dl;King Cr
erB B-2（或HER-2或neu）基因在大约三分之一的乳腺癌、胃癌和卵巢癌中扩增并过表达。越来越多的证据表明，erB B-2过表达与乳腺癌患者生存率降低有关。为了理解erB B-2过表达如何可能赋予乳腺癌细胞更大的恶性潜能，我们寻找了与erB B-2过表达相关的基因表达变化的证据。利用互补DNA文库的差异筛选，我们鉴定了几种代表mRNA的互补DNA，其表达可能根据er B B-2水平而变化。对其中一个互补DNA进行了详细的研究。编码核糖体蛋白L19（1.9个酶）的mRNA在表达高水平er B B-2的乳腺癌样本中更丰富（P < 6 × 10−7）。L19 mRNA的表达水平在肿瘤样品中的变化范围为1- 64倍。未发现L19基因扩增的证据。这些乳腺肿瘤样本中L19的过表达与其他核糖体蛋白（S16和L26）的mRNA表达增加无关。
The erb B-2 (or HER-2 or neu ) gene is amplified and overexpressed in approximately one-third of cancers of the breast, stomach, and ovary. Evidence is accumulating that erb B-2 overexpression is associated with decreased survival of breast cancer patients. In an effort to understand how erb B-2 overexpression might impart a more malignant potential to breast cancer cells, we have searched for evidence of changes in gene expression associated with erb B-2 overexpression. Using differential screening of a complementary DNA library we identified several complementary DNAs that represent mRNAs the expression of which may vary according to erb B-2 level. One complementary DNA was studied in detail. The mRNA encoding the ribosomal protein L19 (1.9 kilobases) was more abundant in breast cancer samples that express high levels of erb B-2 ( P < 6 × 10−7). The level of L19 mRNA expression varied over a 1- to 64-fold range among the tumor samples. No evidence of gene amplification for L19 was identified. The L19 overexpression in these breast tumor samples was not associated with the increased expression of the mRNAs for other ribosomal proteins (S16 and L26).