Identification and profiling of conserved and novel microRNAs from Chinese Qinchuan bovine longissimus thoracis.
Identification and profiling of conserved and novel microRNAs from Chinese Qinchuan bovine longissimus thoracis.
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中国秦川牛胸最长肌保守和新型microRNA的鉴定和分析
DOI：
10.1186/1471-2164-14-42
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发表时间：
2013-01-18
期刊：
影响因子：
4.4
通讯作者：
Chen H
中科院分区：
文献类型：
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作者：
Sun J;Li M;Li Z;Xue J;Lan X;Zhang C;Lei C;Chen H
micrornas （miRNAs）是一个由约22个核苷酸组成的小RNA分子家族，通过完全或部分结合其互补序列来调节基因表达。近年来，大量的mirna及其表达模式在不同的物种中被鉴定出来。然而，到目前为止，还没有mirna调节肉牛肌肉发育的报道。结果利用秦川牛胎儿期和成年期胸最长肌总RNA构建小RNA文库，进行Solexa SBS技术测序。从胎牛文库中共获得15,454,182个clean reads，从成年牛文库中获得13,558,164个clean reads。总共鉴定了521种miRNA，其中包括104种新的miRNA候选物。此外，还分析了已知mirna的核苷酸偏倚、碱基编辑和家族。基于茎环qPCR，检测到25个高读miRNAs，结果显示bta-miRNA-206、miRNA-1、miRNA-133、miRNAn12和miRNAn17在肌肉相关组织或器官中高表达，提示这些miRNAs可能在牛肌肉组织发育中发挥作用。结论本研究证实了已知的417种mirna的真实性，在牛中发现了104种新的mirna，并鉴定出5种肌肉特异性mirna。新mirna的鉴定大大扩展了牛mirna的库，并有助于进一步研究牛的肌肉发育。
BackgroundMicroRNAs (miRNAs) are a family of ~22 nucleotide small RNA molecules that regulate gene expression by fully or partially binding to their complementary sequences. Recently, a large number of miRNAs and their expression patterns have been identified in various species. However, to date no miRNAs have been reported to modulate muscle development in beef cattle.ResultsTotal RNAs from the Chinese Qinchuan bovine longissimus thoracis at fetal and adult stages were used to construct small RNA libraries for Solexa SBS technology sequencing. A total of 15,454,182 clean reads were obtained from the fetal bovine library and 13,558,164 clean reads from the adult bovine library. In total, 521 miRNAs including 104 novel miRNA candidates were identified. Furthermore, the nucleotide bias, base edit and family of the known miRNAs were also analyzed. Based on stem-loop qPCR, 25 high-read miRNAs were detected, and the results showed that bta-miRNA-206, miRNA-1, miRNA-133, miRNAn12, and miRNAn17 were highly expressed in muscle-related tissue or organs, suggesting that these miRNAs may play a role in the development of bovine muscle tissues.ConclusionsThis study confirmed the authenticity of 417 known miRNAs, discovered 104 novel miRNAs in bos taurus, and identified five muscle-specific miRNAs. The identification of novel miRNAs significantly expanded the repertoire of bovine miRNAs and could contribute to further studies on the muscle development of cattle.
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影响因子：
--
作者：
Jin W;Grant JR;Stothard P;Moore SS;Guan LL
通讯作者：
Guan LL
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
DOI：
10.1186/1477-7827-9-40
发表时间：
2011-03-29
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Lingenfelter BM;Tripurani SK;Tejomurtula J;Smith GW;Yao J
通讯作者：
Yao J
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
DOI：
10.1083/jcb.200911036
发表时间：
2010-09-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen JF;Tao Y;Li J;Deng Z;Yan Z;Xiao X;Wang DZ
通讯作者：
Wang DZ