Circadian cycles are the dominant transcriptional rhythm in the intertidal mussel Mytilus californianus
Circadian cycles are the dominant transcriptional rhythm in the intertidal mussel Mytilus californianus
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DOI：
10.1073/pnas.1111076108
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发表时间：
2011-09-20
影响因子：
11.1
通讯作者：
Gracey, Andrew Y.
中科院分区：
文献类型：
--
作者：
Connor, Kwasi M.;Gracey, Andrew Y.
海洋潮间带是陆地和海洋栖息地交汇的地带，居民居住的环境既受陆地24小时昼夜节律的影响，也受潮汐周期12.4小时涨落的影响。在这里，我们调查的相对贡献的日常和潮汐周期的生理潮间带贻贝，贻贝californianus，通过监测基因表达的节奏在模拟和自然潮汐环境。我们报告说，> 40%的转录组表现出节律性基因表达，并且根据特定的潮汐条件，80%至90%的节律性转录物遵循24至26小时的昼夜节律表达模式。与昼夜节律周期的显性效应一致，我们发现时钟基因的表达以24小时为周期振荡。我们的数据表明，昼夜节律的24小时周期是在这个潮间带居民的节奏基因表达的主导驱动程序，尽管在潮汐出现的空气暴露与深刻的环境和生理变化。
Residents in the marine intertidal, the zone where terrestrial and marine habitats converge, inhabit an environment that is subject to both the 24-h day and night daily rhythm of the terrestrial earth and also the 12.4-h ebb and flow of the tidal cycle. Here, we investigate the relative contribution of the daily and tidal cycle on the physiology of intertidal mussels, Mytilus californianus, by monitoring rhythms of gene expression in both simulated and natural tidal environments. We report that > 40% of the transcriptome exhibits rhythmic gene expression, and that depending on the specific tidal conditions, between 80% and 90% of the rhythmic transcripts follow a circadian expression pattern with a period of 24 to 26 h. Consistent with the dominant effect of the circadian cycle we show that the expression of clock genes oscillates with a 24-h period. Our data indicate that the circadian 24-h cycle is the dominant driver of rhythmic gene expression in this intertidal inhabitant despite the profound environmental and physiological changes associated with aerial exposure during tidal emergence.