Litsea Species as Potential Antiviral Plant Sources
Litsea Species as Potential Antiviral Plant Sources
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山苍子物种作为潜在的抗病毒植物来源
DOI：
10.1142/s0192415x16500166
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发表时间：
2016-04-01
影响因子：
5.7
通讯作者：
Zhang, Hongjie
中科院分区：
文献类型：
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作者：
Guan, Yifu;Wang, Dongying;Zhang, Hongjie
山苍子是樟科植物，用于治疗因创伤或蛇咬引起的肿胀，是由美国国立卫生研究院(NIH)资助的国际合作生物多样性组织(ICBG)项目发现的第一种具有抗HIV活性的植物。从该植物中，我们发现了一类新的8个化合物，即典型的倍半萜类化合物，它们都具有抗HIV活性。在随后的研究中，又鉴定了26个不同结构类型的化合物。在对该植物的持续调查中，我们鉴定了两个新的木犀烷，木犀草素L和M，以及一个新的倍半萜丁烯内酯，木犀草素丁烯内酯。L和M具有抑制HIV-1复制的作用，其IC50值为49.6mM。为了进一步确定该植物的抗病毒特性，对另外21个病毒指标进行了比较。木脂素8和木脂素9对EB病毒有抑制作用，其EC50值分别为22.0亩M(SI=3：8)和16.2亩M(SI>；6：2)。鉴于在垂柳中发现了许多抗病毒化合物，我们进一步制备了38种植物提取物，这些提取物是从另外9种山苍子的不同植物部位提取的。对这些提取物进行了抗艾滋病毒和细胞毒活性的评估，其中四种提取物在20微克/毫升的浓度下对一组人类细胞株没有显示出细胞毒性，但对三个不同物种的艾滋病毒复制有97%-100%的抑制作用。
Litsea verticillata Hance (Lauraceae), a Chinese medicine used to treat swelling caused by injury or by snake bites, was the first plant identified by our National Institutes of Health (NIH)-funded International Cooperative Biodiversity Group (ICBG) project to exhibit anti-HIV activities. From this plant, we discovered a class of 8 novel litseane compounds, prototypic sesquiterpenes, all of which demonstrated anti-HIV activities. In subsequent studies, 26 additional compounds of different structural types were identified. During our continuing investigation of this plant species, we identified two new litseanes, litseaverticillols L and M, and a new sesquiterpene butenolide, litseasesquibutenolide. Litseaverticillols L and M were found to inhibit HIV-1 replication, with an IC50 value of 49.6 mu M. To further determine the antiviral properties of this plant, several relatively abundant isolates, including a litseane compound, two eudesmane sesquiterpenes and three lignans, were evaluated against an additional 21 viral targets. Lignans 8 and 9 were shown to be active against the Epstein-Barr Virus (EBV), with EC50 values of 22.0 mu M (SI = 3: 8) and 16.2 mu M (SI > 6: 2), respectively. Since many antiviral compounds have been discovered in L. verticillata, we further prepared 38 plant extracts made from the different plant parts of 9 additional Litsea species. These extracts were evaluated for their anti-HIV and cytotoxic activities, and four of the extracts, which ranged across three different species, displayed 97-100% inhibitory effects against HIV replication without showing cytotoxicity to a panel of human cell lines at a concentration of 20 mu g/mL.