Preliminary Evaluation of the Use of the sefA Fimbrial Gene to Elicit Immune Response Against Salmonella enterica Serotype Enteritidis in Chickens
Preliminary Evaluation of the Use of the sefA Fimbrial Gene to Elicit Immune Response Against Salmonella enterica Serotype Enteritidis in Chickens
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DOI：
10.1637/7438-090905r.1
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发表时间：
2006-06
期刊：
影响因子：
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通讯作者：
V. Lopes;B. Velayudhan;D. Halvorson;K. Nagaraja
中科院分区：
文献类型：
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作者：
V. Lopes;B. Velayudhan;D. Halvorson;K. Nagaraja
摘要近20年来，肠道沙门氏菌（Salmonella enterica serotype enteritrification，简称沙门氏菌）在全球范围内的流行率急剧上升，成为食源性疾病的主要病因，也是一个重要的公共卫生问题。许多研究表明SEF14菌毛蛋白在肠道沙门氏菌粘附宿主中的作用。在本研究中，sefA基因，它编码的主要亚基的SEF14菌毛蛋白，被克隆到一个温度敏感的表达载体，并转化到一个非致病性，无毒的大肠杆菌菌株。将重组菌作为疫苗接种于1日龄雏鸡体内，诱导针对沙门氏菌SefA蛋白的特异性免疫应答。重组菌株被重新从处理的鸟的肠中分离，处理后长达21天，证明其能够定殖1日龄鸡的肠道。此外，针对SefA蛋白的免疫球蛋白A（IgA）在处理后7天的处理鸟的肠分泌物和处理后14至21天的胆汁样品中通过酶联免疫吸附测定法检测。未处理的鸟没有显示出任何证据的肠定植的重组菌株或抗SefA IgA反应，在他们的胆汁或肠分泌物。重组菌株的初步评价表明，该菌株的潜在用途，以引起对沙门氏菌感染的鸡的保护。需要进一步的实验来研究重组菌株保护鸟类免受沙门氏菌定植的能力。
Abstract In the last 2 decades, the prevalence of Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) has dramatically increased worldwide, becoming the leading cause of food-borne illnesses and an important public health issue. Many studies have suggested the role of the SEF14 fimbrial protein in the adhesion of Salmonella Enteritidis to the host. In the present study, the sefA gene, which encodes the main subunit of the SEF14 fimbrial protein, was cloned into a temperature-sensitive expression vector and transformed into a nonpathogenic, avirulent strain of Escherichia coli. The recombinant strain was used as a vaccine to elicit specific immune response against the SefA protein of Salmonella Enteritidis in 1-day-old chickens. The recombinant strain was reisolated from the intestines of treated birds for up to 21 days posttreatment, demonstrating its ability to colonize the intestinal tracts of 1-day-old chickens. In addition, immunoglobulin A (IgA) against the SefA protein was detected in intestinal secretions from treated birds at 7 days posttreatment and in bile samples from 14 to 21 days posttreatment by enzyme-linked immunosorbent assay. Nontreated birds did not show any evidence of intestinal colonization by the recombinant strain or anti-SefA IgA response in their bile or intestinal secretions. Preliminary evaluation of the recombinant strain showed a potential use of this strain to elicit protection against Salmonella Enteritidis infection in chickens. Further experiments are needed to study the ability of the recombinant strain to protect birds against Salmonella Enteritidis colonization.