Identification of inbred strains of mice and genetic relationships between strains as assessed by DNA fingerprinting.
Identification of inbred strains of mice and genetic relationships between strains as assessed by DNA fingerprinting.
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通过 DNA 指纹识别鉴定小鼠近交系以及各系之间的遗传关系。
DOI：
10.1538/expanim1978.42.2_169
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
K. Fukuta
中科院分区：
文献类型：
--
作者：
H. Mannen;S. Tsuji;N. Goto;K. Fukuta
以M13噬菌体DNA为探针，应用DNA指纹图谱技术对近交系小鼠进行了品系鉴定和亲缘关系分析。我们使用了8个近交系（NC/Jah、DDD/Jah、DSD/Jah、RR/Jah、SS/Jah、C3 H/HeJah、C57 BL/6 Jah和C57 BL/6CrSlc）和共基因系（NC-brp）以及同源系（C57 BL/6-bg）。使用M13噬菌体DNA作为探针的DNA指纹显示菌株内的相同模式。没有观察到由于每个菌株内的小卫星区域中的新突变而产生的条带。带型具有菌株特异性。因此，DNA指纹法似乎适合于小鼠的遗传监测。由DNA指纹图谱计算的差值构建的树状图表明，品系之间的关系反映了这些品系的历史，因此DNA指纹图谱也可以有效地用于评估小鼠近交系之间的关系。
Strain identification and the assessment of genetic relationships among inbred strains of mice were examined by DNA fingerprinting using M13 phage DNA as a probe. We used 8 inbred strains (NC/Jah, DDD/Jah, DSD/Jah, RR/Jah, SS/Jah, C3H/HeJah, C57BL/6 Jah and C57BL/6CrSlc) and coisogenic (NC-brp) as well as congenic (C57BL/6-bg) strains. DNA fingerprints using M13 phage DNA as a probe revealed identical patterns within strains. Bands due to new mutations in minisatellite regions within each strain were not observed. The banding patterns were strain-specific. Thus, DNA fingerprinting seemed to be suitable for genetic monitoring of mice. A dendrogram constructed with difference values calculated from DNA fingerprint patterns showed that relationships among strains reflected the history of these strains, so DNA fingerprinting could also be effectively used to assess the relationships between inbred strains of mice.
DOI：
10.1073/pnas.87.7.2496
发表时间：
1990-04
影响因子：
11.1
作者：
H. Reeve;D. Westneat;W. Noon;P. Sherman;C. Aquadro
通讯作者：
H. Reeve;D. Westneat;W. Noon;P. Sherman;C. Aquadro