Reconstitution of contractile FtsZ rings in liposomes
Reconstitution of contractile FtsZ rings in liposomes
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DOI：
10.1126/science.1154520
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发表时间：
2008-05-09
期刊：
影响因子：
56.9
通讯作者：
Erickson, Harold P.
中科院分区：
文献类型：
--
作者：
Osawa, Masaki;Anderson, David E.;Erickson, Harold P.
FtsZ是一种微管蛋白同系物，是细菌细胞分裂中的主要细胞骨架蛋白。它组装成Z环，其中包含FtsZ和十几个其他分裂蛋白，并收缩以分裂细胞。我们通过在其C端剪接一个两亲性螺旋，构建了一个膜靶向的FtsZ（FtsZ-mts）.当与脂质囊泡混合时，FtsZ-mts掺入到一些管状囊泡的内部。在那里，它形成了多个Z环，这些Z环可以沿着脂质体的长度在两个方向上沿着横向移动，并合并成更亮的Z环。较亮的Z环在脂质体中产生可见的收缩，表明FtsZ本身可以组装Z环并产生力。不需要其他蛋白质来组装和产生力。
FtsZ is a tubulin homolog and the major cytoskeletal protein in bacterial cell division. It assembles into the Z ring, which contains FtsZ and a dozen other division proteins, and constricts to divide the cell. We have constructed a membrane- targeted FtsZ ( FtsZ- mts) by splicing an amphipathic helix to its C terminus. When mixed with lipid vesicles, FtsZ- mts was incorporated into the interior of some tubular vesicles. There it formed multiple Z rings that could move laterally in both directions along the length of the liposome and coalesce into brighter Z rings. Brighter Z rings produced visible constrictions in the liposome, suggesting that FtsZ itself can assemble the Z ring and generate a force. No other proteins were needed for assembly and force generation.