Transcriptional Analysis of Mice Melanoma B16-F10 Cells in Response to Directed Current Electric Fields
Transcriptional Analysis of Mice Melanoma B16-F10 Cells in Response to Directed Current Electric Fields
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DOI：
10.1002/bem.22412
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发表时间：
2022-05-31
影响因子：
1.9
通讯作者：
Zhao，Sanjun
中科院分区：
文献类型：
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作者：
Yun，Jinlei;Jin，Xiaoli;Zhao，Sanjun
内源性电场（EF）在组织中广泛存在。它被认为是肿瘤转移的重要环境因素。为了探索内源性EFs在肿瘤转移中的作用，研究了小鼠黑素瘤B16-F10细胞在定向电流EFs（dcEFs）中的迁移。使用RNA测序分析黑素瘤B16-F10细胞响应EF刺激的转录组。结果表明，小鼠黑色素瘤B16-F10细胞在施加的dcEF中向阴极迁移。定向迁移以电压依赖性方式发生。在dcEF下鉴定了大约3000个上调和2613个下调的基因。一些与细胞迁移相关的基因如Serpine 1、Ctgf、Fosb和Fos表达上调。参与细胞运动的信号通路被显著改变。一些与肿瘤发生、侵袭和转移高度相关的基因响应于EF刺激而上调。内源性EFs可能在体内肿瘤的发生和转移中发挥作用。© 2022生物电磁学学会。
The endogenous electric field (EF) is widely observed among tissues. It is supposed to be an important environmental factor in tumor metastasis. To explore the role of endogenous EFs in tumor metastasis, the migration of mouse melanoma B16‐F10 cells in directed current EFs (dcEFs) was investigated. The transcriptome of melanoma B16‐F10 cells in response to EF stimulation was analyzed using RNA sequencing. The results demonstrated that the mouse melanoma B16‐F10 cells migrated toward the cathode in applied dcEFs. Directional migration occurred in a voltage‐dependent manner. Approximately 3000 upregulated and 2613 downregulated genes were identified under dcEF. Some genes correlated with cell migration, such asSerpine1, Ctgf, Fosb, andFos, were upregulated. The signaling pathways involved in cell motility were significantly altered. Some genes, highly related to tumorigenesis, invasion, and metastasis, are upregulated in response to EF stimulation. Endogenous EFs may play a role in tumorigenesis and metastasis in vivo. © 2022 Bioelectromagnetics Society.