STUDIES ON ORIGIN OF PANDEMIC INFLUENZA .2. PEPTIDE MAPS OF LIGHT AND HEAVY POLYPEPTIDE CHAINS FROM HEMAGGLUTININ SUBUNITS OF A2 INFLUENZA-VIRUSES ISOLATED BEFORE AND AFTER APPEARANCE OF HONG-KONG INFLUENZA
STUDIES ON ORIGIN OF PANDEMIC INFLUENZA .2. PEPTIDE MAPS OF LIGHT AND HEAVY POLYPEPTIDE CHAINS FROM HEMAGGLUTININ SUBUNITS OF A2 INFLUENZA-VIRUSES ISOLATED BEFORE AND AFTER APPEARANCE OF HONG-KONG INFLUENZA
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DOI：
10.1016/0042-6822(72)90055-4
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发表时间：
1972-01-01
期刊：
影响因子：
3.7
通讯作者：
WEBSTER, RG
中科院分区：
文献类型：
--
作者：
LAVER, WG;WEBSTER, RG
从1968年香港流感大流行发生前获得的三株A2/亚洲流感病毒株，以及1968年、1970年和1971年在世界不同地区分离的三株香港流感病毒株中分离出血凝素亚单位。用盐酸胍和二硫苏糖醇分离血凝素亚基，离心分离重、轻多肽链。然后用胰蛋白酶消化每个分离的多肽链，并绘制胰蛋白酶肽图谱。这些图谱显示，1968年分离的“老”a2病毒的血凝素亚基的多肽链与香港毒株的多肽链在氨基酸序列上有很大不同。在轻链和重链中都出现了显著的差异。另一方面，三个香港轻链的图谱相似，而重链的图谱除了一两个多肽外，其他都是相同的。A2/亚洲1968菌株的图谱在许多肽链（轻链和重链）上存在差异，但与香港菌株的图谱没有任何相似之处。这些结果显示，香港流感的血凝素亚基并不是由先前存在的人类a2毒株突变而来的，更合理的解释是，它们可能来自动物或禽流感病毒的基因重组。
Hemagglutinin subunits were isolated from three strains of A2/Asian influenza obtained in 1968 before the occurrence of the Hong Kong influenza pandemic, and from three strains of Hong Kong influenza isolated in different parts of the world in 1968, 1970 and 1971. The hemagglutinin subunits were dissociated by treatment with guanidine hydrochloride and dithiothreitol and the light and heavy polypeptide chains were separated by centrifugation. Each of the isolated polypeptide chains was then digested with trypsin and the tryptic peptides were mapped. The maps showed that the polypeptide chains from the hemagglutinin subunits of the “old” A2viruses isolated in 1968 differed greatly in amino acid sequence from the polypeptide chains of the Hong Kong strains. Striking differences occurred in both light and heavy chains. On the other hand, the maps of the three Hong Kong light chains were similar and the maps of the heavy chains were identical with the exception of one or two peptides only. The maps from the three A2/Asian 1968 strains differed in a number of peptides (both in the light and heavy chains) but none bore any resemblance to the maps of the Hong Kong strains.These results suggest that the hemagglutinin subunits of Hong Kong influenza were not derived by mutation from a preexisting human A2strain: it is thought that a more reasonable explanation for their origin is that they came, probably by genetic recombination, from an animal or avian influenza virus.