Isolation and characterization of plant growth promoting endophytic bacteria and their effect on tomato (Lycopersicon esculentum) and chilli (Capsicum annuum) seedling growth
Isolation and characterization of plant growth promoting endophytic bacteria and their effect on tomato (Lycopersicon esculentum) and chilli (Capsicum annuum) seedling growth
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DOI：
10.1007/s13213-011-0321-7
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发表时间：
2012-06-01
影响因子：
3
通讯作者：
Thajuddin, Nooruddin
中科院分区：
文献类型：
--
作者：
Amaresan, Natarajan;Jayakumar, Velusamy;Thajuddin, Nooruddin
已知存在于植物组织中的内生细菌经常被证明促进植物生长。本研究涉及八个假定的内生菌分离物的作物植物番茄和辣椒的表面消毒种子的效力。2株分离株为革兰氏阴性，6株为革兰氏阳性。所有分离物能够在高达6%NaCl中生长，并且分离物BETL13在高达12%NaCl中生长。使用微生物鉴定系统（Biolog）鉴定分离株。在8个分离株中，6个属于芽孢杆菌属，2个属于沙雷氏菌属。BECS 7、BECS 14的16S rRNA基因部分序列与Bacillus sp.的相似性分别为99%和98%，BECS 11与Bacillus subtilis的相似性为97%，BECS 1与Arthrobacter sp.的相似性为98%。辣椒炭疽病菌和腐霉内生菌也被发现产生吲哚乙酸和铁载体和4个菌株溶解无机磷酸盐。在温室条件下，通过辣椒和番茄的生物测定证明了植物生长促进特性。分离物BETL13、BETL9、BECS 1、BECL 8和BECS 7在根和芽长度以及相对于其宿主的次生根数量的增加方面以及在交叉接种中显示出植物生长促进作用。
The endophytic bacteria known to reside in plant tissues have often been shown to promote plant growth. The present study deals with the efficacy of eight isolates of putative endophytes from surface- sterilized seeds of the crop plants tomato and chilli. Two isolates were Gram negative and six were Gram positive. All isolates were able to grow in up to 6% NaCl, and isolate BETL13 grew in up to 12% NaCl. The isolates were identified using a microbial identification system (Biolog). Of the eight isolates, six belong to the genus Bacillus and two are Serratia. The partial 16S rRNA gene sequences of BECS7, BETS14 shared 99% and 98% similarity with Bacillus sp. BETS11 showed 97% similarity to Bacillus subtilis and BECS1 showed 98% similarity to Arthrobacter sp. Under in vitro conditions, all eight isolates showed antagonistic activity against Sclerotium rolfsii, Fusarium oxysporum, Colletotrichum capsici and Pythium sp. The endophytes were also found to produce indole acetic acid and siderophore and four isolates solubilized inorganic phosphate. The plant growth promotion properties were demonstrated through a chilli- and tomato- based bioassay under greenhouse conditions. Isolates BETL13, BETL9, BECS1, BECL8 and BECS7 showed plant growth promotion in terms of an increase in root and shoot length and the number of secondary roots with respect to their host as well as in cross inoculation.