Global analysis of circadian expression in the cyanobacterium Synechocystis sp strain PCC 6803
Global analysis of circadian expression in the cyanobacterium Synechocystis sp strain PCC 6803
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DOI：
10.1128/jb.187.6.2190-2199.2005
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发表时间：
2005-03-01
影响因子：
3.2
通讯作者：
Ishiura, M
中科院分区：
文献类型：
--
作者：
Kucho, K;Okamoto, K;Ishiura, M
蓝细菌是唯一被发现有生物钟的细菌物种。我们使用DNA微阵列来研究蓝细菌集胞藻菌株PCC 6803的昼夜表达模式。我们的分析确定了54（2%）和237（9%）的基因，表现出昼夜节律的严格和宽松的过滤条件下，分别。大多数循环基因的表达在从主观的白天到夜晚的过渡时间附近达到峰值，这表明集胞藻生物钟的主要作用是调整细胞的生理状态以适应即将到来的夜晚环境。有几个染色体区域，其中相邻基因以相似的昼夜节律模式表达。循环基因的生理功能是多样的，包括各种各样的代谢途径，膜运输和信号转导。参与呼吸和聚3-羟基烷酸酯合成的基因表现出协调的昼夜表达，表明这种调控对于夜间能量和碳源的供应是重要的。参与转录和翻译的基因也遵循昼夜循环模式。这些基因可能对生物钟产生的节律信息的输出很重要。我们的研究结果为生物钟对细胞生理学的重要性和时钟控制的基因调控机制提供了重要的见解。
Cyanobacteria are the only bacterial species found to have a circadian clock. We used DNA microarrays to examine circadian expression patterns in the cyanobacterium Synechocystis sp. strain PCC 6803. Our analysis identified 54 (2%) and 237 (9%) genes that exhibited circadian rhythms under stringent and relaxed filtering conditions, respectively. The expression of most cycling genes peaked around the time of transition from subjective day to night, suggesting that the main role of the circadian clock in Synechocystis is to adjust the physiological state of the cell to the upcoming night environment. There were several chromosomal regions where neighboring genes were expressed with similar circadian patterns. The physiological functions of the cycling genes were diverse and included a wide variety of metabolic pathways, membrane transport, and signal transduction. Genes involved in respiration and poly(3-hydroxyalkanoate) synthesis showed coordinated circadian expression, suggesting that the regulation is important for the supply of energy and carbon source in the night. Genes involved in transcription and translation also followed circadian cycling patterns. These genes may be important for output of the rhythmic information generated by the circadian clock. Our findings provided critical insights into the importance of the circadian clock on cellular physiology and the mechanism of clock-controlled gene regulation.