Novel nano-device to measure voltage-driven membrane transporter activity
Novel nano-device to measure voltage-driven membrane transporter activity
复制标题
测量电压驱动膜转运蛋白活性的新型纳米装置
DOI：
10.1109/tnano.2015.2498167
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发表时间：
2016
影响因子：
2.4
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Watanabe;R.;Soga;N.;& Noji;H.
使用排列脂质双层腔室系统（ALBiC）可以对膜转运蛋白活性进行高度敏感的定量分析，这是药物研究的主要目标。虽然膜电压是转运蛋白的主要驱动力之一，但由于电压调制的技术限制，ALBiC的多功能性仅限于没有膜电压的转运蛋白分析。在这里，我们报告了一种基于ALBiC （el-ALBiC）的新型纳米器件，该器件含有亚百万脂质双层腔，每个腔都配备了纳米尺寸的电极。由于纳米级电极能够对膜电压进行定量调制，el-ALBiC能够对电压驱动的膜转运蛋白活性进行高灵敏度检测。因此，新型纳米装置el-ALBiC扩展了ALBiC的多功能性，并具有进一步分析和药理学应用的潜力，例如药物筛选。
The use of an arrayed lipid bilayer chamber system (ALBiC) enables highly sensitive quantitative analysis of membrane transporter activity, a major target of pharmaceutical research. Although membrane voltage is one of the main driving forces of transporters, the versatility of ALBiC is limited to transporter assays in the absence of membrane voltage, owing to technical limitations with voltage modulation. Here, we report a novel nano-device based on ALBiC (el-ALBiC) containing sub-million lipid bilayer chambers, each equipped with nano-sized electrodes. Since the nano-sized electrodes enable quantitative modulation of membrane voltage, the el-ALBiC is capable of performing highly sensitive detection of the voltage-driven membrane transporter activity. Thus, the novel nano-device el-ALBiC extends the versatility of ALBiC and has potential for further analytical and pharmacological applications, such as drug screening.