HIV type 2 protease, reverse transcriptase, and envelope viral variation in the PBMC and genital tract of ARV-naive women in Senegal.
HIV type 2 protease, reverse transcriptase, and envelope viral variation in the PBMC and genital tract of ARV-naive women in Senegal.
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塞内加尔未接受过抗逆转录病毒治疗的妇女的 PBMC 和生殖道中的 HIV 2 型蛋白酶、逆转录酶和包膜病毒变异。
DOI：
10.1089/aid.2008.0015
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发表时间：
2008
影响因子：
1.5
通讯作者：
Sow,PapaSalif
中科院分区：
文献类型：
--
作者：
Gottlieb,GeoffreyS;Hawes,StephenE;Wong,KimG;Raugi,DanaN;Agne,HabibatouD;Critchlow,CathyW;Kiviat,NancyB;Sow,PapaSalif
未感染 HIV-2 ARV 的女性的生殖道中可能会出现独特的病毒变异和耐药突变。我们对来自塞内加尔四名未接受过抗逆转录病毒药物的妇女的 PBMC 和生殖道样本的蛋白酶 (PR)、逆转录酶 (RT) 和包膜 (ENV) 进行了测序和系统发育分析。预测 HIV-1 蛋白酶抑制剂 (PI) 耐药性的 HIV-2 蛋白酶多态性很常见。两名受试者的生殖道样本中存在蛋白酶突变（T77I 和 I64V），但在 PBMC 中未发现这种突变。一名受试者在 CVL DNA（但不是 PBMC）中存在 HIV-2 逆转录酶 M184I 突变，已知该突变会赋予 HIV-2 3TC/FTC 耐药性。在另一名受试者中，CVL-RNA 中也发现了逆转录酶 A62V 突变，但 PBMC 中未发现。我们发现 PBMC 和生殖道之间的 ENV 变异没有显着差异。未接受过 ARV 治疗的女性生殖道中的 HIV-2 RT 和 PR 突变可能对传播的 HIV-2 耐药性和 ARV 治疗产生影响。
Unique viral variants and resistance mutations may occur in the genital tract of HIV-2 ARV-naive infected women. We sequenced and phylogenetically analyzed protease (PR), reverse transcriptase (RT), and envelope (ENV) from PBMC and genital tract samples from four ARV-naive women in Senegal. HIV-2 protease polymorphisms that predict HIV-1 protease inhibitor (PI) resistance were common. Two subjects had protease mutations (T77I and I64V) in genital tract samples that were not found in PBMCs. One subject had the HIV-2 reverse transcriptase M184I mutation in CVL DNA (but not PBMCs) that is known to confer 3TC/FTC resistance in HIV-2. In another subject, the reverse transcriptase A62V mutation was also found in CVL-RNA but not PBMCs. We found no significant difference in ENV variants between PBMCs and the genital tract. HIV-2 RT and PR mutations in the genital tract of ARV-naive females may have implications for transmitted HIV-2 resistance and ARV therapy.