The Effects of ASMase Mediated Endothelial Cell Apoptosis in Multiple Hypofractionated Irradiations in CT26 Tumor Bearing Mice.
The Effects of ASMase Mediated Endothelial Cell Apoptosis in Multiple Hypofractionated Irradiations in CT26 Tumor Bearing Mice.
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DOI：
10.7314/apjcp.2015.16.11.4543
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发表时间：
2015-06
期刊：
影响因子：
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通讯作者：
Hong Zhu;Kai Deng;Yaqin Zhao;Xin Wang;Yali Shen;Tai-guo Liu;D. Cui;Feng Xu
中科院分区：
文献类型：
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作者：
Hong Zhu;Kai Deng;Yaqin Zhao;Xin Wang;Yali Shen;Tai-guo Liu;D. Cui;Feng Xu
背景：探讨ASMase介导的CT26荷瘤小鼠内皮细胞凋亡在多次低分割照射中的作用。材料与方法35只CT26荷瘤小鼠分别接受0、3、6、9、12、15、18 Gy的单次电离辐射。IR后8 h，处死小鼠，用肿瘤组织进行CD31免疫组化染色、TUNEL和CD31双染色、ASMase活性测定。然后根据上述结果选择6 Gy和12 Gy进行多次减分红外实验。每次IR后，处死5只小鼠，如上所述。结果单次辐照12 Gy及以上后，ASMase活性显著升高，内皮细胞凋亡明显增加，MVD明显降低。在未达到触发ASMase激活的6 Gy条件下，2次及以上辐照后，ASMase活性显著升高，内皮细胞凋亡显著增加，MVD显著降低。而在12 Gy时，2次及以上辐照后，ASMase活性维持不变，内皮细胞凋亡率无明显升高；然而，MVD明显降低。6 Gy和12 Gy多次辐照后，肿瘤细胞凋亡率均显著升高。结论ASMase介导的内皮细胞凋亡可能在CT26结直肠癌多次低分割IR过程中发挥重要作用。
BACKGROUND To investigate the effects of ASMase mediated endothelial cell apoptosis in multiple hypofractionated irradiations in CT26 tumor bearing mice. MATERIALS AND METHODS Thirty-five CT26 tumor bearing mice were subjected to single ionizing radiation (IR) of 0, 3, 6, 9, 12, 15, 18 Gy. Eight hours after IR, the mice were sacrificed and tumor tissues were used for CD31 immunohistochemistry staining, TUNEL and CD31 double staining, ASMase activity assay. Then 6 and 12 Gy were chosen for multiple hypofractionated IR experiments according to the above results. Each time after IR, 5 mice were sacrificed and assayed as above. RESULTS The ASMase activities were increased significantly after a single IR of 12 Gy or higher which was accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. For 6 Gy which was not high enough to trigger ASMase activation, after 2 or more times of IR, the ASMase activities were significantly increased accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. While for 12 Gy, after 2 or more times of IR, the ASMase activities and endothelial cell apoptosis rates were maintained without remarkable increase; however, the MVD was significantly decreased. What's more, the cancer cell apoptosis rates were significantly increased after multiple IR for both 6 Gy and 12 Gy. CONCLUSIONS ASMase mediated endothelial cell apoptosis may play an important role in the process of multiple hypofractionated IR for CT26 colorectal carcinoma.