Structure-antioxidant relationships of sulfated galactomannan from guar gum.
Structure-antioxidant relationships of sulfated galactomannan from guar gum.
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DOI：
10.1016/j.ijbiomac.2009.10.004
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发表时间：
2010
影响因子：
8.2
通讯作者：
Xiaofang Wang;Junlong Wang;Ji Zhang;Baotang Zhao;Jian Yao;Yunpu Wang
中科院分区：
文献类型：
--
作者：
Xiaofang Wang;Junlong Wang;Ji Zhang;Baotang Zhao;Jian Yao;Yunpu Wang
硫酸化多糖具有潜在的生物活性，其活性与硫酸化程度、分子量、取代位置和链构象有关。本文对商品瓜尔胶进行了提纯，并合成了不同取代度和分子量的瓜尔胶硫酸酯衍生物。FT-IR和13 C NMR分析表明，硫酸化产物中C-6取代占主导地位。在硫酸化反应中，观察到MW急剧下降。df值在1.92 ~ 2.85之间，表明-SO 3 H基团的引入导致了硫酸化多糖构象的相对膨胀。抗氧化实验表明硫酸酯化多糖具有较好的抗氧化活性。在体外模型中获得的数据表明，高DS和低MW显示出最好的抗氧化能力。
Sulfated polysaccharides exerted potential biological property which was relative to degree of sulfation (DS), Mw, substitution position and chain conformation. In the present study, commercial guar gum was purified and its sulfated derivates with different DS and Mwwere synthesized. FT-IR and13C NMR analysis indicated that C-6 substitution was predominant in sulfated samples compared with other positions. In the sulfation reaction, a sharp decrease in Mwwas observed. The dfvalues from 1.92 to 2.85 indicated that the –SO3H groups led to the relatively expanded conformation of sulfated polysaccharides. Antioxidant assays showed that sulfated polysaccharides had better antioxidant activities. The data obtained in in vitro models indicated that high DS and low Mwshowed the best antioxidant capacities.