Honeybee gut microbiota promotes host weight gain via bacterial metabolism and hormonal signaling
Honeybee gut microbiota promotes host weight gain via bacterial metabolism and hormonal signaling
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DOI：
10.1073/pnas.1701819114
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发表时间：
2017-05-02
影响因子：
11.1
通讯作者：
Moran, Nancy A.
中科院分区：
文献类型：
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作者：
Zheng, Hao;Powell, J. Elijah;Moran, Nancy A.
社会蜜蜂拥有一个简单而专门的微生物群，在空间上组织成不同的肠道区室。最近关于蜜蜂肠道群落在病原体保护和营养功能中的潜在参与的结果引起了人们对微生物群对蜜蜂健康的影响的关注。然而，肠道微生物群对宿主生理的贡献还有待研究。在这里，我们表明肠道微生物群促进个体蜜蜂全身和肠道的体重增加。这种效应可能是由宿主卵黄蛋白原、胰岛素信号和味觉反应的变化介导的。我们发现，微生物代谢通过产生短链脂肪酸显著降低肠道pH值和氧化还原电位，并且邻近肠壁的细菌在肠道内形成氧梯度。短链脂肪酸谱的优势肠道物种的贡献在体外得到证实。此外，代谢组学分析表明，肠道群落对肠道隔室和血淋巴的代谢特征具有显著影响，表明肠道细菌降解花粉中的植物聚合物，所得代谢产物有助于宿主营养。我们的研究结果表明微生物代谢如何影响蜜蜂的生长，激素信号，行为和肠道理化条件。这些发现表明，蜜蜂肠道微生物群具有与其他一些动物相似的基本作用，因此为宿主-微生物相互作用的研究提供了模型。
Social bees harbor a simple and specialized microbiota that is spatially organized into different gut compartments. Recent results on the potential involvement of bee gut communities in pathogen protection and nutritional function have drawn attention to the impact of the microbiota on bee health. However, the contributions of gut microbiota to host physiology have yet to be investigated. Here we show that the gut microbiota promotes weight gain of both whole body and the gut in individual honey bees. This effect is likely mediated by changes in host vitellogenin, insulin signaling, and gustatory response. We found that microbial metabolism markedly reduces gut pH and redox potential through the production of short-chain fatty acids and that the bacteria adjacent to the gut wall form an oxygen gradient within the intestine. The short-chain fatty acid profile contributed by dominant gut species was confirmed in vitro. Furthermore, metabolomic analyses revealed that the gut community has striking impacts on the metabolic profiles of the gut compartments and the hemolymph, suggesting that gut bacteria degrade plant polymers from pollen and that the resulting metabolites contribute to host nutrition. Our results demonstrate how microbial metabolism affects bee growth, hormonal signaling, behavior, and gut physicochemical conditions. These findings indicate that the bee gut microbiota has basic roles similar to those found in some other animals and thus provides a model in studies of host-microbe interactions.