HIGH-PERFORMANCE LIQUID AFFINITY CHROMATOGRAPHY (HPLAC) AND ITS APPLICATION TO SEPARATION OF ENZYMES AND ANTIGENS
HIGH-PERFORMANCE LIQUID AFFINITY CHROMATOGRAPHY (HPLAC) AND ITS APPLICATION TO SEPARATION OF ENZYMES AND ANTIGENS
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DOI：
10.1016/0014-5793(78)80792-3
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发表时间：
1978-01-01
期刊：
影响因子：
3.5
通讯作者：
MOSBACH, K
中科院分区：
文献类型：
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作者：
OHLSON, S;HANSSON, L;MOSBACH, K
2.材料和方法结晶LADH（1.85 U/mg）、#-NADH（III级）、、&NAD（III级）、BSA（结晶）和AMP购自Sigma（密苏里州圣路易斯）。猪心脏 (400 U/mg) 和兔肌肉 (550 U/mg) 的 LDH 由 Boehringer (Mannheim) 提供，缩写：LDH，乳酸脱氢酶；LADH，马肝乙醇脱氢酶；BSA，牛血清白蛋白；HSA，人血清白蛋白；aufs，满量程吸收单位
2. Materials and methodsCrystalline LADH (1.85 U/mg),#-NADH (grade III),, &NAD (grade III), BSA (crystalline) and AMP were purchased from Sigma (St. Louis, MO.). LDH from pig heart (400 U/mg) and rabbit muscle (550 U/mg) were supplied by Boehringer (Mannheim, Abbreviations: LDH, Lactate dehydrogenase; LADH, Horse liver alcohol dehydrogenase; BSA, Bovine serum albumin; HSA, Human serum albumin; aufs, Absorption units of full scale