Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution
Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution
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DOI：
10.1073/pnas.0401077101
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发表时间：
2004-05-18
影响因子：
11.1
通讯作者：
Tsutsumi, N
中科院分区：
文献类型：
--
作者：
Arimura, S;Yamamoto, J;Tsutsumi, N
线粒体融合和分裂之间的平衡影响酵母线粒体的网状形状。关于植物中是否发生线粒体融合，人们知之甚少。植物线粒体通常比动物线粒体数量更多，形状更像颗粒。BLAST搜索拟南芥的核和线粒体基因组序列，没有发现任何明显的同源物的线粒体融合基因在动物和酵母中发现。为了确定线粒体融合是否真的发生在植物中，我们用一种以洋葱为靶点的光转换荧光蛋白Kaede标记了洋葱表皮细胞中的线粒体，然后将细胞内一些线粒体的荧光从绿色改变为红色。红色和绿色线粒体的频繁和短暂融合通过随后重新分裂的黄色线粒体的出现来证明。我们还表明，线粒体分裂偶尔会发生，而没有一个平等的分布的类核（DNA-蛋白质复合物在线粒体），导致在一个单一的细胞内含有不同数量的DNA的线粒体的共存。线粒体内DNA含量的不均一性可通过线粒体的频繁而短暂的融合来克服。
The balance between mitochondrial fusion and fission influences the reticular shape of mitochondria in yeasts. Little is known about whether mitochondria fusion occurs in plants. Plant mitochondria are usually more numerous and more grain-shaped than animal mitochondria. BLAST searches of the nuclear and mitochondrial genome sequences of Arabidopsis thaliana did not find any obvious homologue of mitochondrial fusion genes found in animals and yeasts. To determine whether mitochondrial fusion actually occurs in plants, we labeled mitochondria in onion epidermal cells with a mitochondria-targeted, photoconvertible fluorescent protein Kaede and then altered the fluorescence of some of the mitochondria within a cell from green to red. Frequent and transient fusion of red and green mitochondria was demonstrated by the appearance of yellow mitochondria that subsequently redivided. We also show that mitochondrial fission occasionally occurs without an equal distribution of the nucleoid (DNA-protein complex in mitochondria), resulting in the coexistence of mitochondria containing various amounts of DNA within a single cell. The heterogeneity of DNA contents in mitochondria may be overcome by the frequent and transient fusion of mitochondria.