Linkage between cooperative oxygenation and subunit assembly of cobaltous human hemoglobin.
Linkage between cooperative oxygenation and subunit assembly of cobaltous human hemoglobin.
复制标题
协同氧合与含钴人血红蛋白亚基组装之间的联系。
DOI：
10.1021/bi00243a031
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Ackers,GK
中科院分区：
文献类型：
--
作者：
Doyle,ML;Speros,PC;LiCata,VJ;Gingrich,D;Hoffman,BM;Ackers,GK
材料和方法制备方法。钴血红蛋白的制备方法如前所述(Hoffman&Peting，1970)。用Beckman SP-5PW离子交换柱进行高效液相色谱分离纯化，经分析等电聚焦证明为均一组分。人结合珠蛋白是用Connell和Shaw(1961)的方法纯化的。
Materials and MethodsPreparations. Cobaltous hemoglobin was prepared as described previously (Hoffman & Petering, 1970). Purification was done by HPLC on a Beckman SP-5PW ion-exchange column and shown to be homogeneousby analytical isoelectric focusing. Human haptoglobin was purified by the method of Connell and Shaw (1961).