Roe-derived phospholipid administration enhances lymphatic docosahexaenoic acid-containing phospholipid absorption in unanesthetized rats
Roe-derived phospholipid administration enhances lymphatic docosahexaenoic acid-containing phospholipid absorption in unanesthetized rats
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DOI：
10.1016/j.plefa.2017.06.011
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发表时间：
2018-12-01
影响因子：
3
通讯作者：
Ohkubo, Takeshi
中科院分区：
文献类型：
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作者：
Murota, Kaeko;Takagi, Mika;Ohkubo, Takeshi
血浆n-3脂肪酸作为n-3脂肪酸向包括脑在内的各种组织的供应库是重要的，尽管膳食n-3脂肪酸与其在血浆中的分子种类之间的关系尚未完全阐明。我们研究了肠道吸收的二十二碳六烯酸（DHA）来自鱼卵磷脂（Roe-PL），并比较它与鱼油甘油三酯和游离DHA使用未麻醉的淋巴管大鼠。来自脑内给药的三个样品的DHA吸收没有显著差异，而Roe-PL给药导致磷脂部分中的DHA水平显著高于其他两个样品给药。DHA在Roe-PL中的sn-2位被胰酶水解的程度低于体外纯化的磷脂酶A2，同时给予游离DHA和溶血磷脂酰胆碱不会产生与Roe-PL给药相同的结果。我们的研究结果表明，膳食中含有DHA的磷脂是有效的，以增加系统的DHA纳入磷脂通过肠道吸收和生物合成。
Plasma n-3 fatty acids are important as the supplying pool of n-3 fatty acids to various tissues including the brain, although the relationship between dietary n-3 fatty acids and their molecular species in the plasma are not fully clarified. We investigated the intestinal absorption of docosahexaenoic acid (DHA) derived from fish roe phospholipid (Roe-PL) and compared it with fish oil triacylglycerol and free DHA using unanesthetized lymph-cannulated rats. The DHA absorption from intraduodenally administered three samples were not significantly different, whereas Roe-PL administration resulted in a significantly higher level of DHA in the phospholipid fraction than the other two samples administrations. DHA in Roe-PL at the sn-2 position was less hydrolyzed by pancreatin than by purified phospholipase A2 in vitro and simultaneous administration of free DHA and lysophosphatidylcholine did not produce the same results as the Roe-PL administration. Our results indicate that dietary DHA-containing phospholipid is effective to increase the systemic DHA incorporated into phospholipids via intestinal absorption and biosynthesis.