Flow-cytometric evaluation of oxidative burst in phagocytic cells of children with cystic fibrosis
Flow-cytometric evaluation of oxidative burst in phagocytic cells of children with cystic fibrosis
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DOI：
10.1159/000024022
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发表时间：
1998-12-01
影响因子：
2.8
通讯作者：
Wolf, H
中科院分区：
文献类型：
--
作者：
Fr端hwirth, M;Ruedl, C;Wolf, H
目的：本研究的目的是评估染料2',7'-二氯荧光素（DCF）检测对囊性纤维化（CF）患者多形核细胞（PMN）和单核细胞（MC）氧化爆发改变的筛查作用。研究设计：56例CF患者，年龄在2 - 20岁之间。纯化后的细胞分别用磷肉豆酸酯（PMA）和酶生酶（ZX）刺激。根据60例健康对照的数据，建立了PMA和zx刺激和非刺激细胞的DCF荧光范围。结果：PMNs表现为增强和损伤。在14例CF患者中检测到由静息细胞异常高的平均荧光强度（MFl）引起的氧化爆发缺陷。6例CF患者氧化破裂增强。CF患者对PMA或ZX刺激的反应不同。铜绿假单胞菌定殖显著增强(p
Objectives: The objective of this study was to assess the dye 2',7'-dichlorofluorescein (DCF assay in screening for alterations in polymorphonuclear cell (PMN) and monocyte (MC) oxidative burst of cystic fibrosis (CF) patients. Study design: 56 CF patients aged between 2 and 20 years were investigated. Purified cells were stimulated with phorbolmyristate acetate: (PMA) and zymosan (ZX). A range for DCF fluorescence for PMA- and ZX-stimulated and non-stimulated cells was established based on data from 60 healthy controls. Results: PMNs showed both enhancement and impairment. A deficient oxidative burst was detected in a total of 14 CF patients caused by abnormally high mean fluorescence intensity (MFl) of resting cells. Enhanced oxidative burst was seen in 6 CF patients. CF patients responded differently to PMA or ZX stimulation. Pseudomonas aeruginosa colonization significantly enhanced (p