Human Bocavirus infection and respiratory tract disease identified in a UK patient cohort
Human Bocavirus infection and respiratory tract disease identified in a UK patient cohort
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DOI：
10.1016/j.jcv.2020.104453
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发表时间：
2020-08-01
影响因子：
8.8
通讯作者：
McClure, C. Patrick
中科院分区：
文献类型：
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作者：
Bagasi, Arwa A.;Howson-Wells, Hannah C.;McClure, C. Patrick
背景资料：自2005年首次分离以来，人类博卡病毒（HBoV）已多次与急性呼吸道感染相关，但由于合并感染率高，其致病作用仍不清楚。目的：评估2015年至2019年期间英国东米德兰兹郡呼吸道患者队列中的HBoV患病率和相关疾病。研究设计：我们最初使用内部PCR检测在2015年至2017年期间采样的回顾性儿科队列中研究了未诊断的HBoV负担。HBoV随后被纳入标准呼吸道诊断途径，我们审计了2018年至2019年期间HBoV阳性结果的日历年。结果：我们对青少年患者先前常规诊断阴性样本的回顾性PCR筛查发现了9%（n= 30）的HBoV 1型患病率。这些明显的皿oVl单一感染经常与呼吸道症状相关，通常严重需要通气、氧气和类固醇干预，31%（n= 9）的个体需要重症监护。当HBoV筛查随后被纳入常规呼吸道诊断途径时，在儿童和成人中观察到全年感染，并在2月份达到高峰。9098人中检出HBoV阳性185人（2.03%），以12-24月龄儿童为主。然而，在60岁以上的患者中也观察到HBoV感染，主要是单一感染。23%的185个独特的患者是HBoV单感染和持续低水平的DNA阳性观察到15个人长达6个月后，最初presentation.Conclusion：HBoV 1是一种流行的呼吸道感染，在英国能够引起严重的单感染。
Background: Since its first isolation in 2005, Human Bocavirus (HBoV) has been repeatedly associated with acute respiratory tract infections, although its role in pathogenicity remains unclear due to high co-infection rates.Objectives: To assess HBoV prevalence and associated disease in a cohort of respiratory patients in the East Midlands, UK between 2015 and 2019.Study design: We initially investigated the undiagnosed burden of HBoV in a retrospective paediatric cohort sampled between 2015 and 2017 using an in-house PCR assay. HBoV was subsequently incorporated into the standard respiratory diagnostic pathway and we audited a calendar year of HBoV positive results between 2018 and 2019.Results: Our retrospective PCR screening of previously routine diagnostic-negative samples from juvenile patients identified a 9% (n= 30) prevalence of HBoV type 1. These apparent HBoV1 mono-infections were frequently associated with respiratory tract symptoms, often severe requiring ventilation, oxygen and steroid intervention with 31% (n= 9) of individuals requiring intensive care. When HBoV screening was subsequently adopted into the routine respiratory diagnostic pathway, year-round infections were observed in both children and adults peaking in February. 185 of 9098 (2.03%) individuals were found to be HBoV positive with children aged 12-24 months the principally infected group. However, HBoV infection was also observed in patients aged over 60, predominantly as a mono-infection. 23% of the 185 unique patients were HBoV monoinfected and persistent low-level DNA positivity was observed in 15 individuals up to 6-months after initial presentation.Conclusion: HBoV1 is a prevalent respiratory infection in the UK capable of causing serious monoinfections.