Microbial community dynamics in a submerged fixed bed bioreactor during biological treatment of saline urban wastewater
Microbial community dynamics in a submerged fixed bed bioreactor during biological treatment of saline urban wastewater
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DOI：
10.1016/j.ecoleng.2014.07.025
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发表时间：
2014-10-01
影响因子：
3.8
通讯作者：
Gonzalez-Lopez, J.
中科院分区：
文献类型：
--
作者：
Cortes-Lorenzo, C.;Sipkema, D.;Gonzalez-Lopez, J.
采用DGGE法和pcr扩增16S核糖体RNA基因片段的454焦磷酸测序法，研究了盐（NaCl）对城市污水处理的浸没式固定床生物反应器系统中细菌和古细菌群落的影响。DGGE指纹图谱聚类分析显示，不同盐分浓度下，群落结构存在显著差异。所有实验均发现变形菌门为优势菌门，低盐度条件下α -变形菌门为主要目，高盐度条件下γ -变形菌门为优势目。在所有实验中，Euryarchaeota是主要的古细菌门，所有微生物都对应于产甲烷古细菌。细菌的α多样性随着盐度的增加而降低，而古细菌的α多样性随着NaCl浓度的增加而增加。(C) 2014 Elsevier B.V.版权所有
The influence of salt (NaCl) on bacterial and archaeal communities in a submerged fixed bed bioreactor system for the treatment of urban wastewater was determined by DGGE and 454 pyrosequencing of PCR-amplified 16S ribosomal RNA gene fragments. Cluster analysis of DGGE fingerprints showed significant differences in the community structure dependent on the salt concentration in the influent. Proteobacteria was found to be the dominant bacterial phylum in all experiments, with alpha-Proteobacteria being the main order at low salinity and gamma-Proteobacteria the dominant order at high salinity. Euryarchaeota was the main archaeal phylum in all experiments, with all microorganisms corresponding to methanogenic archaea. Whereas bacterial alpha-diversity decreased as salinity increased, archaeal alpha-diversity increased with higher NaCl concentrations. (C) 2014 Elsevier B.V. All rights reserved.