Metabolic response of longitudinal muscles to acute hypoxia in sea cucumber Apostichopus japonicus (Selenka): A metabolome integrated analysis
Metabolic response of longitudinal muscles to acute hypoxia in sea cucumber Apostichopus japonicus (Selenka): A metabolome integrated analysis
复制标题
海参纵向肌肉对急性缺氧的代谢反应：代谢组学综合分析
DOI：
10.1016/j.cbd.2018.12.007
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发表时间：
2019-03-01
影响因子：
3
通讯作者：
Storey, Kenneth B.
中科院分区：
文献类型：
--
作者：
Li, Li;Chen, Muyan;Storey, Kenneth B.
低氧是世界范围内严重的水环境问题，几十年来已导致海参(及其他物种)大量死亡，严重影响了水产养殖业的可持续发展。研究海参在急性低氧胁迫下的代谢紊乱和生化反应，可为提高水产养殖水平提供理论依据和指导。一种代谢组学的方法来表征内源性小分子在急性缺氧时的变化，可以帮助确定代谢损伤的主要潜在原因，并可能提出缓解的解决方案，以提高生存能力。本研究采用液-质联用(LC-MS)和多元分析方法，研究了日本对虾在急性低氧应激条件下(分别为实验组EG6和EG24)和对照组(n=10)在急性低氧应激(以氮气曝气的方式在2分钟内将水族箱水中的溶解氧降至2 mg/L)后纵肌的代谢变化。结果表明，EG6和EG24中分别有29种和62种代谢产物受到显著影响，主要包括脂类、糖苷及其衍生物。大多数脂质(脂肪酸、甘油脂、甘油磷脂、鞘脂和甾醇)水平在两个实验组均升高，并且随着低氧时间的延长而增加，表明低氧应激对脂质及其衍生物的合成和降解的动态平衡产生了强烈的影响。4(脂肪酸生物合成、D-谷氨酰胺和D-谷氨酸代谢、糖酵解/糖异生、乙醛酸和二羧酸代谢)和2(类固醇生物合成、长寿调节途径)分别在EG6和EG24中显著富含。这些结果表明，两个处理组的脂肪酸合成均显著增强，且EG24组的程度高于EG6组，为了解日本对虾对低氧胁迫的特殊适应机制提供了有价值的信息。
Hypoxia is a severe problem in aquatic environments worldwide and has caused mass mortality of sea cucumbers (& other species) for decades, seriously affecting the sustainable development of aquaculture. Investigations of the metabolic disruptions and biochemical responses associated with acute hypoxia stress in sea cucumbers can provide a theoretical basis and guidance for improving aquaculture. A metabolomics approach to characterize changes in the profiles of endogenous small molecules in response to acute hypoxia can help to identify the main underlying causes of metabolic damage and potentially suggest solutions to alleviate to improve viability. The current study uses liquid chromatography-mass spectrometry (LC-MS) and multivariate analysis methods to evaluate the metabolic profile of longitudinal muscles from A. japonicas exposed to acute hypoxia stress (by bubbling the aquaria water with nitrogen aeration to decrease dissolved oxygen to 2 mg/L in 2 min) for 6 or 24 h (experimental groups EG6 or EG24) and control group (CG, n = 10, respectively). The results showed that 29 and 62 metabolites were influenced significantly in EG6 and EG24, respectively, mainly including lipids, glycosides and their derivatives. Levels of most lipids (fatty acids, glycerolipids, glycerophospholipids, sphingolipids and sterols) were elevated in both experimental groups, and increased with elongation of hypoxia, implying that the homeostasis of synthesis and degradation of lipids and their derivatives was strongly affected by hypoxia stress. Pathway enrichment analysis was performed to further assess the importance of differential metabolite expression to the development of the A. japonicus response to hypoxia, showing that 4 (fatty acid biosynthesis, D-glutamine and D-glutamate metabolism, glycolysis/gluconeogenesis, glyoxylate and dicarboxylate metabolism) and 2 (steroid biosynthesis, longevity regulating pathway) pathways were markedly enriched in EG6 and EG24, respectively. These results suggested that fatty acid synthesis was strengthened significantly in both treatment groups, and the degree was higher in EG24 than in EG6, providing valuable information towards understanding the special adaptive mechanism of A. japonicus to hypoxia stress.