MAMMALIAN GALACTOKINASE. DEVELOPMENTAL AND ADAPTIVE CHARACTERISTICS IN THE RAT LIVER.
MAMMALIAN GALACTOKINASE. DEVELOPMENTAL AND ADAPTIVE CHARACTERISTICS IN THE RAT LIVER.
复制标题
哺乳动物半乳糖激酶。
DOI：
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发表时间：
1965
影响因子：
4.8
通讯作者：
S. Segal
中科院分区：
文献类型：
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作者：
P. Cuatrecasas;S. Segal
半乳糖激酶催化三磷酸腺苷和n-半乳糖形成cy-n-半乳糖l-磷酸（1，2），是半乳糖进入葡萄糖代谢不同途径的一系列酶中的第一种酶（3，4）。虽然半乳糖激酶已在各种微生物中进行了广泛研究，但在哺乳动物组织中的研究仅限于确认其存在于肝、脑和红细胞中（3，5-7）。已经进行了哺乳动物半乳糖激酶的研究，以评估该酶在半乳糖代谢调节中的参与，特别是在对饮食半乳糖有关键依赖的年轻人中。目前的工作描述了年轻和成年大鼠肝半乳糖激酶的某些特性，并建议存在至少两种不同的肝酶能够磷酸化半乳糖。半乳糖激酶活性也在其他几个大鼠组织中进行了测量。描述了发育过程中发生的变化和饮食治疗引起的变化。
Galactokinase catalyzes the formation of cy-n-galactose l-phosphate from adenosine triphosphate and n-galactose (1, 2) and is the first enzyme in a series by which galactose enters the varied pathways of glucose metabolism (3, 4). Although galactokinase has been studied extensively in various microorganisms, in mammalian tissues investigations have been limited to confirmation of its presence in liver, brain, and erythrocytes (3, 5-7). Studies of mammalian galactokinase have been undertaken in order to evaluate the participation of the enzyme in the regulation of galactose metabolism, especially in the young in which there is a critical dependence on dietary galactose. The present work describes certain properties of young and adult rat liver galactokinase and suggests the presence of at least two different hepatic enzymes capable of phosphorylating galactose. Galactokinase activity was also measured in several other rat tissues. Changes occurring during development and those induced by dietary treatment are described.