Budding yeast complete DNA synthesis after chromosome segregation begins
Budding yeast complete DNA synthesis after chromosome segregation begins
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DOI：
10.1038/s41467-020-16100-3
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发表时间：
2020-05-08
影响因子：
16.6
通讯作者：
Mendoza, Manuel
中科院分区：
文献类型：
--
作者：
Ivanova, Tsvetomira;Maier, Michael;Mendoza, Manuel
为了忠实地传递遗传信息，细胞必须在分裂前复制其整个基因组。这被认为是通过复制和染色体分离的时间分离来保证的。在20-40%未受干扰的酵母细胞中，DNA合成在有丝分裂后期继续进行。高cyclin-Cdk活性抑制中期的DNA合成，有丝分裂退出期cyclin-Cdk活性的降低使DNA合成在亚端粒和一些难以复制的区域完成。有丝分裂后期的DNA合成与亚端粒区域突变率升高相关，包括拷贝数变异。因此，酵母细胞在正常生长期间暂时重叠DNA合成和染色体分离，可能允许细胞最大化群体水平的生长速度，同时探索更大的遗传空间。在细胞周期的S期，完整的基因组需要在细胞分裂发生之前被复制。在这里，作者表明，在芽殖酵母DNA合成完成后，染色体分离开始。
To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that in 20-40% of unperturbed yeast cells, DNA synthesis continues during anaphase, late in mitosis. High cyclin-Cdk activity inhibits DNA synthesis in metaphase, and the decrease in cyclin-Cdk activity during mitotic exit allows DNA synthesis to finish at subtelomeric and some difficult-to-replicate regions. DNA synthesis during late mitosis correlates with elevated mutation rates at subtelomeric regions, including copy number variation. Thus, yeast cells temporally overlap DNA synthesis and chromosome segregation during normal growth, possibly allowing cells to maximize population-level growth rate while simultaneously exploring greater genetic space. In the S phase of the cell cycle, the full genome needs to be replicated before cell division occurs. Here, authors show that in budding yeast DNA synthesis is completed after chromosome segregation begins.