Plasma membrane as a site of redox activation of daunomycin in intact human erythrocytes. Quantitative evaluation of the hydrogen peroxide produced by the membrane with respect to the cytosol.
Plasma membrane as a site of redox activation of daunomycin in intact human erythrocytes. Quantitative evaluation of the hydrogen peroxide produced by the membrane with respect to the cytosol.
复制标题
质膜作为完整人红细胞中道诺霉素氧化还原激活的位点。
DOI：
10.1016/0006-2952(92)90469-y
复制
发表时间：
1992
影响因子：
5.8
通讯作者：
G. Rotilio
中科院分区：
文献类型：
--
作者：
L. Marcocci;P. Pietrangeli;I. Mavelli;G. Rotilio
通过测定接触道诺霉素时产生的 H2O2 来评估与血红蛋白相比，人红细胞质膜作为蒽环类药物氧化还原激活位点的相对重要性。将3-氨基-1,2,4-三唑依赖H2O2不可逆抑制过氧化氢酶(EC 1.11.1.6)活性的方法应用于完整红细胞以及添加纯化过氧化氢酶的分离膜。获得的结果表明，从定量的角度来看，道诺霉素在质膜激活中起着次要作用，尽管当考虑到细胞质抗氧化防御的高效率和膜激活位点的外部位置时，膜途径可能比细胞质途径更有害，特别是对于细胞外靶标。
The relative importance in human red blood cells of the plasma membrane as a site of redox activation of anthracyclines as compared to hemoglobin was evaluated by assaying the H2O2produced upon exposure to daunomycin. The method of H2O2-dependent irreversible inhibition of catalase (EC 1.11.1.6) activity by 3-amino-1,2,4-triazole was applied to intact erythrocytes, as well as to isolated membranes with added purified catalase. The results obtained indicate a secondary role in daunomycin activation for the plasma membrane from a quantitative point of view, although membrane pathways can be more harmful than cytosolic pathways, especially towards extracellular targets, when the high efficiency of the cytosolic antioxidative defences and the external location of the membrane activation site are considered.