Effects of putrescine on synthesis and degradation of ornithine decarboxylase in primary cultured hepatocytes.
Effects of putrescine on synthesis and degradation of ornithine decarboxylase in primary cultured hepatocytes.
复制标题
腐胺对原代培养肝细胞中鸟氨酸脱羧酶合成和降解的影响。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
S. Hayashi
中科院分区：
文献类型：
--
作者：
R. Kanamoto;K. Utsunomiya;T. Kameji;S. Hayashi
在原代培养的成年大鼠肝细胞中，观察了门冬酰胺和胰高血糖素诱导鸟氨酸脱羧酶（ODC）的过程中ODC合成速率和降解速率的变化，以及腐胺引起ODC快速降解的过程。ODC的合成速率通过[35 S]蛋氨酸掺入酶中来测定，随后通过免疫沉淀和十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳来分离。通过跟踪预标记ODC的衰变来确定ODC的降解速率。由天冬酰胺（10 mM）和胰高血糖素（1 μ M）引起的酶诱导是由于合成速率的增加和降解速率的降低。添加10 mM腐胺引起ODC活性的快速衰减，这比在放线菌酮存在下的ODC衰减更快。这种ODC活性的快速衰减伴随着ODC蛋白的略微较慢的衰减，这是由于ODC合成的部分抑制和ODC降解的几倍加速。
Changes in both synthesis rate and degradation rate of ornithine decarboxylase (ODC) were pursued in primary cultures of adult rat hepatocytes during the process of ODC induction caused by asparagine and glucagon and also during the process of rapid ODC decay caused by putrescine. The synthesis rate of ODC was determined by [35S]methionine incorporation into the enzyme, which was separated afterwards by immunoprecipitation and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The degradation rate of ODC was determined by following the decay of prelabeled ODC. The enzyme induction caused by asparagine (10 mM) and glucagon (1 microM) was due both to an increase in the synthesis rate and to a decrease in the degradation rate. Addition of 10 mM putrescine caused a rapid decay of ODC activity, which was faster than ODC decay in the presence of cycloheximide. This rapid decay in ODC activity was accompanied by slightly slower decay in ODC protein, which was due both to partial suppression of ODC synthesis and to several fold acceleration of ODC degradation.
小鼠肾脏鸟氨酸脱羧酶蛋白的结构和合成速率的研究。
影响因子：
2.9
作者：
Persson,L;Seely,JE;Pegg,AE
通讯作者：
Pegg,AE
1,3-二氨基丙烷对硫代乙酰胺处理的大鼠肝脏中鸟氨酸脱羧酶蛋白的影响。
DOI：
10.1042/bj2160701
发表时间：
1983
期刊：
The Biochemical journal
影响因子：
--
作者：
Seely,JE;Pegg,AE
通讯作者：
Pegg,AE
二环己胺对 HTC 细胞多胺含量和鸟氨酸脱羧酶活性的影响。
DOI：
10.1016/0304-4165(85)90210-7
发表时间：
1985
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Mitchell,JL;Mahan,DW;McCann,PP;Qasba,P
通讯作者：
Qasba,P