DETECTION OF HOMOEOLOGOUS CHIASMA FORMATION IN TRITICUM-DURUM X THINOPYRUM-BESSARABICUM HYBRIDS USING GENOMIC IN-SITU HYBRIDIZATION
DETECTION OF HOMOEOLOGOUS CHIASMA FORMATION IN TRITICUM-DURUM X THINOPYRUM-BESSARABICUM HYBRIDS USING GENOMIC IN-SITU HYBRIDIZATION
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DOI：
10.1038/hdy.1993.151
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发表时间：
1993-10-01
期刊：
影响因子：
3.8
通讯作者：
MILLER, TE
中科院分区：
文献类型：
--
作者：
KING, IP;PURDIE, KA;MILLER, TE
用基因组原位杂交(GISH)技术研究了硬粒小麦(Triticum hard um cv.)Creso，ph1c突变纯合子。小麦/小麦和小麦/Th的相对频率。Bessarabicum chiasma的形成。配对明显不同的Th。此外，还观察了Bessarabicum的染色体。讨论了GISH作为分析小麦/异源杂交种同源交叉形成的工具的潜力。
Genomic in situ hybridization (GISH) was used to study the nature of homoeologous chiasma formation in crosses between Triticum durum cv. Creso, homozygous for the ph1c mutation and Thinopyrum bessarabicum. The relative frequencies of wheat/wheat and wheat/Th. bessarabicum chiasma formation were determined. Pairing between apparently non-homologous Th. bessarabicum chromosomes was also observed. The potential of GISH as a tool for analysing homoeologous chiasma formation in wheat/alien hybrids is discussed.