Role of bovine serum albumin and humic acid in the interaction between SiO2 nanoparticles and model cell membranes.
Role of bovine serum albumin and humic acid in the interaction between SiO2 nanoparticles and model cell membranes.
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DOI：
10.1016/j.envpol.2016.09.059
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发表时间：
2016-12
影响因子：
8.9
通讯作者：
Xiaoran Wei;Xiaolei Qu;Lei Ding;Jingtian Hu;Wei Jiang
中科院分区：
文献类型：
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作者：
Xiaoran Wei;Xiaolei Qu;Lei Ding;Jingtian Hu;Wei Jiang
二氧化硅纳米颗粒（SiO2 NPs）在释放到环境中后会对健康造成危害。天然有机物和生物分子在SiO2纳米颗粒上的吸附改变了它们的表面性质和细胞毒性。本研究采用牛血清白蛋白（BSA）和腐殖酸（HA）对SiO2纳米粒子进行处理，研究其对模型细胞膜完整性和流动性的影响。为了避免细胞活性的干扰，制备了巨单层囊泡和小单层囊泡（GUV和SUV）作为模型细胞膜。显微镜下观察发现，BSA/HA处理的（BSA-/HA-）SiO2 NPs比未处理的SiO2 NPs需要更长的时间来破坏膜，这是因为BSA/HA吸附覆盖了表面的SiSingle键OH/SiSingle键O-基团，削弱了NPs与磷脂之间的相互作用。通过石英晶体微天平（QCM-D）监测SiO2 NPs在膜上的沉积。未经处理的和HA-SiO2纳米颗粒迅速破坏了QCM-D传感器上的SUV层; BSA-SiO2纳米颗粒附着在膜上，但仅引起缓慢的囊泡破坏。未处理的-，BSA-和HA-SiO2纳米粒子都引起了凝胶化的带正电荷的膜，这是由广义极性值进行评估。HA-SiO_2纳米粒子引起的凝胶化最严重，BSA-SiO_2纳米粒子引起的凝胶化最少。我们的研究结果表明，蛋白质吸附在SiO2纳米粒子减少了NP引起的膜损伤。
Silica nanoparticles (SiO2NPs) can cause health hazard after their release into the environment. Adsorption of natural organic matter and biomolecules on SiO2NPs alters their surface properties and cytotoxicity. In this study, SiO2NPs were treated by bovine serum albumin (BSA) and humic acid (HA) to study their effects on the integrity and fluidity of model cell membranes. Giant and small unilamellar vesicles (GUVs and SUVs) were prepared as model cell membranes in order to avoid the interference of cellular activities. The microscopic observation revealed that the BSA/HA treated (BSA-/HA-) SiO2NPs took more time to disrupt membrane than untreated-SiO2NPs, because BSA/HA adsorption covered the surface Sisingle bondOH/Sisingle bondO-groups and weakened the interaction between NPs and phospholipids. The deposition of SiO2NPs on membrane was monitored by a quartz crystal microbalance with dissipation (QCM-D). Untreated- and HA-SiO2NPs quickly disrupted the SUV layer on QCM-D sensor; BSA-SiO2NPs attached on the membranes but only caused slow vesicle disruption. Untreated-, BSA- and HA-SiO2NPs all caused the gelation of the positively-charged membrane, which was evaluated by the generalized polarity values. HA-SiO2NPs caused most serious gelation, and BSA-SiO2NPs caused the least. Our results demonstrate that the protein adsorption on SiO2NPs decreases the NP-induced membrane damage.