Bacillus amyloliquefaciens BNM122, a potential microbial biocontrol agent applied on soybean seeds, causes a minor impact on rhizosphere and soil microbial communities
Bacillus amyloliquefaciens BNM122, a potential microbial biocontrol agent applied on soybean seeds, causes a minor impact on rhizosphere and soil microbial communities
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DOI：
10.1016/j.apsoil.2008.10.007
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发表时间：
2009-02-01
影响因子：
4.8
通讯作者：
Garcia, Augusto F.
中科院分区：
文献类型：
--
作者：
Correa, Olga S.;Montecchia, Marcela S.;Garcia, Augusto F.
大豆产量的提高促使更多地使用农用化学品，而农用化学品造成了重大问题，如土壤和水污染以及生物多样性减少，并对非目标物种产生了负面影响。微生物生物防治的发展。大豆病害防治剂有助于减少农药滥用。解淀粉芽孢杆菌BNM 122是一种很有潜力的生防微生物。在植物生长室和温室中，当接种到大豆种子中时，能够控制立枯丝核菌引起的立枯病的药剂。本文报道了用BNM 122菌株或两种杀菌剂（福美双和多菌灵）处理大豆种子对大豆根际细菌群落结构和功能的影响。此外，大豆根瘤菌的根瘤化，由丛枝菌根真菌和植物生长进行了评价。我们使用r-和K-策略的概念，以评估可培养的细菌群落的生理生态结构，在Biolog（TM）EcoPlates的社区水平的生理概况（CLPP），以研究细菌的功能，和模式的16 S RNA基因的PCR扩增和分离的变性梯度凝胶电泳（PCR-DGGE），以评估细菌群落的遗传结构。接种BNM 122后，大豆根际细菌群落的生理生态结构和生理特性均未发生明显变化。与此相反，种子处理杀菌剂增加的比例r-战略家和改变根际可培养细菌群落的代谢概况。根际细菌群落的遗传结构在处理和未处理的种子之间没有表现出明显的变化。对于细菌和真菌的共生，种子处理不影响大豆结瘤，而大豆菌根化在用菌株BNM 122或用杀菌剂处理的种子获得的植物中显著降低（P < 0.05）。然而，在用杀真菌剂处理种子的植物中观察到更高的负面效应。种子处理对植株生长无明显影响，说明B处理大豆种子对植株生长有促进作用。解淀粉芽孢杆菌BNM 122对土壤微生物群落的影响小于杀菌剂，这可能是由于该菌株的环境持久性和毒性较小所致，值得进一步研究以开发商业化制剂。(C)2008 Elsevier B. V.保留所有权利。
The increase in soybean productivity has contributed to a greater use of agrochemicals, which cause major problems, such as soil and water pollution and reduction of biodiversity, and have a negative impact on non-target species. The development of microbial biocontrol. agents for soybean diseases can help to reduce pesticide abuse. Bacillus amyloliquefaciens BNM122 is a potential microbial biocontrol. agent able to control the damping-off caused by Rhizoctonia solani when inoculated in soybean seeds, both in a plant growth chamber and in a greenhouse. in this study, we report the effect of soybean seed treatments with strain BNM122 or with two fungicides (thiram and carbendazim) on the structure and function of the bacterial community that colonizes the soybean rhizosphere. Also, soybean root nodulation by Bradyrhizobium japonicum, mycorrhization by arbuscular mycorrhizal fungi and plant growth were evaluated. We used the r- and K-strategist concept to evaluate the ecophysiological structure of the culturable bacterial community, community-level physiological profiles (CLPP) in Biolog (TM) EcoPlates to study bacterial functionality, and the patterns of 16S RNA genes amplified by PCR and separated by denaturing gradient gel electrophoresis (PCR-DGGE) to assess the genetic structure of the bacterial community. Neither the ecophysiological structure nor the physiological profiles of the soybean rhizosphere bacterial community showed important changes after seed inoculation with strain BNM122. On the contrary, seed treatment with fungicides increased the proportions of r-strategists and altered the metabolic profiles of the rhizosphere culturable bacterial community. The genetic structure of the rhizosphere bacterial community did not show perceptible changes between treated and non-treated seeds. Regarding the bacterial and fungal symbioses, seed treatments did not affect soybean nodulation, whereas soybean mycorrhization significantly decreased (P < 0.05) in plants obtained from seeds treated with strain BNM122 or with the fungicides. However, a higher negative effect was observed in plants which seeds were treated with the fungicides. Plant growth was not affected by seed treatments.It can be concluded that soybean seed treatment with B. amyloliquefaciens BNM122 had a lesser effect on soil microbial community than that with the fungicides, and that these differences may be attributed to the less environmental persistence and toxic effects of the strain, which deserve further studies in order to develop commercial formulations. (C) 2008 Elsevier B.V. All rights reserved.