Interaction of metastasis associated Mts1 protein with nonmuscle myosin.
Interaction of metastasis associated Mts1 protein with nonmuscle myosin.
复制标题
作者：
Heide L. Ford;Sayeeda Zain
mts1基因编码一个101个氨基酸的蛋白质，属于S100 Ca（2+）结合蛋白亚家族，与非转移性癌相比，在转移性癌中过表达。虽然Mts1蛋白质是puronecular参与细胞膜相互作用，其确切的生理作用是未知的。为了深入了解Mts1的生物学功能，在整个小鼠胚胎发生以及正常成年小鼠组织中监测其表达。小鼠胚胎的原位杂交显示，MTS1 mRNA在8天大的胚胎的滋养层细胞中表达最高。在正常成年小鼠组织中，免疫细胞化学显示Mts1在胸腺和脾脏中以T细胞特异性模式表达。这些结果表明，MTS1的表达是普遍的运动细胞类型，并进一步阐明MTS 1的作用，凝胶覆盖和GST融合蛋白的实验进行，以确定与MTS 1蛋白相互作用的蛋白质。发现非肌肉肌球蛋白II与Mts1相互作用，表明Mts1在运动过程中的作用，并证实了Mts1可能通过增加癌细胞的运动性来增强转移潜能的假设。
The mts1 gene codes for a 101 amino acid protein which belongs to the subfamily of S100 Ca(2+)-binding proteins and is overexpressed in metastatic cancers as compared to their nonmetastatic counterparts. While the Mts1 protein is putatively involved in cytoskeletal-membrane interactions, its exact physiological role is not known. In order to gain insight into the biological function of Mts1, its expression was monitored throughout mouse embryogenesis as well as in normal adult mouse tissues. In situ hybridizations of mouse embryos showed expression of mts1 mRNA to be highest in trophoblast cells of a day 8 old embryo. In normal adult mouse tissues, immunocytochemistry revealed Mts1 expression in a T-cell specific pattern in both the thymus and spleen. These results indicate that the expression of mts1 is prevalent in motile cell types, and to further elucidate the role of Mts1, gel overlay and GST-fusion protein experiments were performed to identify proteins that interact with the Mts1 protein. Nonmuscle myosin II was found to interact with Mts1, suggesting a role for Mts1 in the process of motility and lending credence to the hypothesis that Mts1 may enhance metastatic potential by increasing the motility of cancerous cells.