Characterization of a rhesus monkey calicivirus representing a new genus of Calicivitidae
Characterization of a rhesus monkey calicivirus representing a new genus of Calicivitidae
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DOI：
10.1128/jvi.00070-08
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发表时间：
2008-06-01
影响因子：
5.4
通讯作者：
Jiang, Xi
中科院分区：
文献类型：
--
作者：
Farkas, Tibor;Sestak, Karol;Jiang, Xi
本研究报告了从杜兰国家灵长类动物研究中心（Tulane National Primate Research Center）圈养的幼年猕猴（Macaca mulatta）粪便样本中分离出的新型钙病毒（Calicivirus，CV）--杜兰病毒（Tulane virus，TV）的特征。TV的完整基因组包含6,714个核苷酸和一个聚（A）尾，由三个开放阅读框（ORE）组成，分别编码非结构（NS）多聚蛋白（ORFI）、纤壳蛋白（ORF2）（估计分子量为57.9 kDa）和一个可能的次结构蛋白（ORF3）（等电点（pI）为10.0，计算分子量为22.8 kDa）。NS 多聚蛋白显示了所有典型的 CV 氨基酸基序，包括 GXXGXGKT（NTPase）、EYXEX（Vpg）、GDCG（蛋白酶）以及 GLPSG 和 YGDD（聚合酶）。根据NS多聚蛋白、NTP酶、蛋白酶、聚合酶和囊膜蛋白序列构建的系统发生树一致将TV置于与诺罗病毒同根的分支上，但其距离与其他属之间的距离相同。TV 可以在猴肾细胞系（LLC-MK2）中培养，并出现典型的细胞病理效应。TV 具有典型的 CV 形态，直径为 36 纳米，浮力密度为 1.37 克/毫升。根据这些理化和遗传特征，TrV 代表了一种新的 CV 属，我们建议将其命名为 "Recovirus"（恒河猴肠道 CV）。尽管TV在猕猴中的致病性仍有待阐明，但TV引起肠道感染的可能性和组织培养系统的可用性使这种病毒成为人类CV的一种有价值的替代物。
In this study, we report the characterization of a novel calicivirus (CV), the Tulane virus (TV), which was isolated from stool samples of captive juvenile rhesus macaques (Macaca mulatta) of the Tulane National Primate Research Center. The complete genome of TV contains 6,714 nucleotides plus a poly(A) tail and is organized into three open reading frames (OREs) that encode the nonstructural (NS) polyprotein (ORFI); the capsid protein (ORF2), with an estimated molecular mass of 57.9 kDa; and a possible minor structural protein (ORF3), with an isoelectric point (pI) of 10.0 and a calculated molecular mass of 22.8 kDa. The NS polyprotein revealed all typical CV amino acid motifs, including GXXGXGKT (NTPase), EYXEX (Vpg), GDCG (protease), and GLPSG and YGDD (polymerase). Phylogenetic trees constructed for the NS polyprotein, NTPase, protease, polymerase, and capsid protein sequences consistently placed the TV on a branch rooted with Norovirus, but with distances equal to those between other genera. The TV can be cultured in a monkey kidney cell line (LLC-MK2) with the appearance of typical cytopathic effect. TV exhibits a typical CV morphology, with a diameter of 36 nm, and has a buoyant density of 1.37 g/ml. According to these physicochemical and genetic characteristics, TrV represents a new CV genus for which we propose the name "Recovirus" (rhesus enteric CV). Although the pathogenicity of TV in rhesus macaques remains to be elucidated, the likelihood of TV causing intestinal infection and the availability of a tissue culture system make this virus a valuable surrogate for human CVs.