Surface characterization and assessment of cell attachment capabilities of thin films fabricated by ion-beam irradiation of poly(l-lactic acid) substrates
Surface characterization and assessment of cell attachment capabilities of thin films fabricated by ion-beam irradiation of poly(l-lactic acid) substrates
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聚（L-乳酸）基底离子束辐照制备薄膜的表面表征和细胞附着能力评估
DOI：
10.1016/j.apsusc.2012.10.196
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发表时间：
2013
影响因子：
6.7
通讯作者：
and Hirofumi Yajima
中科院分区：
文献类型：
--
作者：
Toshiyuki Tanaka;Yoshiaki Suzuki;Koji Tsuchiya;and Hirofumi Yajima
聚（L-乳酸）（PLLA），一种可生物降解的聚合物，基板的离子束照射，产生剥离薄膜时，基板浸入水溶液中。该薄膜表现出优异的细胞亲和性，因此，可用于生物工程应用。在这项研究中，我们的特点是两个表面的薄膜，并评估其细胞附着能力。通过X射线光电子能谱（XPS）和动态力显微镜（DFM）分析每个表面。这些分析表明，碳化发生在两个表面。此外，在两个表面的形貌中未观察到显著变化。最后，通过在其上培养小鼠成纤维细胞来确定表面的细胞附着能力。细胞牢固地附着于膜的底部以及顶部表面，并且良好地展开。这些结果可以归因于薄膜表面的碳化。这种薄膜，通过生物可降解聚合物的辐射制造，预计将在组织再生和细胞移植等领域找到广泛的应用。
The ion-beam irradiation of substrates of poly(l-lactic acid) (PLLA), a biodegradable polymer, gave rise to exfoliatable thin films when the substrate was immersed in an aqueous solution. The thin films exhibited excellent cell affinity, and hence, can be useful in bioengineering applications. In this study, we characterized both surfaces of the thin films and evaluated their cell attachment capabilities. Each surface was analyzed by X-ray photoelectron spectroscopy (XPS) and dynamic force microscopy (DFM). These analyses showed that carbonization took place at both surfaces. In addition, no significant changes were noticed in the topographies of the two surfaces. Finally, the cell attachment capabilities of the surfaces were determined by culturing mouse fibroblasts on them. The cells attached firmly to the bottom as well as the top surface of the film and were well spread out. These results could be attributed to the carbonization of the surfaces of the thin-film. Such thin films, fabricated by the irradiation of a biodegradable polymer, are expected to find wide application in areas such as tissue regeneration and cell transplantation.