Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite
Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite
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DOI：
10.1002/elan.201000235
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发表时间：
2010-11
期刊：
影响因子：
3
通讯作者：
Wei Wang;Hong-Yi Li;De-Wen Zhang;Jingjing Jiang;Yi-Ran Cui;Shuang Qiu;Ying Zhou;Xinxiang Zhang
中科院分区：
文献类型：
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作者：
Wei Wang;Hong-Yi Li;De-Wen Zhang;Jingjing Jiang;Yi-Ran Cui;Shuang Qiu;Ying Zhou;Xinxiang Zhang
探索了金纳米颗粒-细菌纤维素纳米纤维（Au-BC）纳米复合材料作为安培测定葡萄糖的平台。将葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）两种酶同时固定在Au-BC纳米复合材料修饰玻碳电极上。在电子介体 (HQ) 存在的情况下，观察到对葡萄糖的灵敏且快速的电流响应。 GOx和HRP在Au-BC纳米复合材料中都保持了很好的生物催化活性。优化条件下葡萄糖的检测限低至 2.3 µM，线性范围为 10 µM 至 400 µM。该生物传感器已成功应用于人体血液样本中葡萄糖的测定。
An exploration of gold nanoparticles–bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 µM with a linear range from 10 µM to 400 µM. The biosensor was successfully applied to the determination of glucose in human blood samples.