Resistant and Susceptible Responses in Tomato to Cyst Nematode are Differentially Regulated by Salicylic Acid
Resistant and Susceptible Responses in Tomato to Cyst Nematode are Differentially Regulated by Salicylic Acid
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DOI：
10.1093/pcp/pcq109
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发表时间：
2010-09-01
影响因子：
4.9
通讯作者：
Masuta, Chikara
中科院分区：
文献类型：
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作者：
Uehara, Taketo;Sugiyama, Shunpei;Masuta, Chikara
为了了解机制的番茄品种窝藏英雄A基因对胞囊线虫使用微阵列，我们首先分析了番茄基因表达马铃薯胞囊线虫（PCN; Globodera rostochiensis）在早期不相容和相容的相互作用接种后3和7天（dpi）。苯丙氨酸解氨酶（PAL）和Myb相关基因的转录水平在3dpi时在不亲和互作中上调。丙酮酸脱羧酶（PDC）和乙醇脱氢酶（ADH）基因的转录也在3dpi的不相容相互作用中上调。而PAL、Myb、PDC和ADH 4个基因在亲和互作中表达下调。通过比较几种病程相关（PR）蛋白基因在非亲和和亲和互作中的表达水平，发现水杨酸（SA）依赖的PR基因在3dpi的非亲和互作中被诱导表达。与未接种的对照相比，PR-1（P4）转录本在孢囊线虫感染的抗性植株中在3dpi时增加到异常高的水平。在不相容和相容的相互作用中，游离SA水平升高到相似的水平。然后，我们证实，PR-1（P4）没有显着诱导的NahG番茄窝藏英雄A基因，与抗性品种相比。因此，我们发现PR-1（P4）是Hero A对PCN赋予的品种抗性的标志，线虫寄生导致敏感品种中SA信号通路的抑制。
To understand the machinery underlying a tomato cultivar harboring the Hero A gene against cyst nematode using microarrays, we first analyzed tomato gene expression in response to potato cyst nematode (PCN; Globodera rostochiensis) during the early incompatible and compatible interactions at 3 and 7 days post-inoculation (dpi). Transcript levels of the phenylalanine ammonia lyase (PAL) and Myb-related genes were up-regulated at 3dpi in the incompatible interaction. Transcription of the genes encoding pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) was also up-regulated at 3dpi in the incompatible interaction. On the other hand, the four genes (PAL, Myb, PDC and ADH) were down-regulated in the compatible interaction at 3dpi. When the expression levels of several pathogenesis-related (PR) protein genes in tomato roots were compared between the incompatible and compatible interactions, the salicylic acid (SA)-dependent PR genes were found to be induced in the incompatible interaction at 3dpi. The PR-1(P4) transcript increased to an exceptionally high level at 3dpi in the cyst nematode-infected resistant plants compared with the uninoculated controls. The free SA levels were elevated to similar levels in both incompatible and compatible interactions. We then confirmed that PR-1(P4) was not significantly induced in the NahG tomato harboring the Hero A gene, compared with the resistant cultivar. We thus found that PR-1(P4) was a hallmark for the cultivar resistance conferred by Hero A against PCN and that nematode parasitism resulted in the inhibition of the SA signaling pathway in the susceptible cultivars.